Experimental Warming Decreases the Average Size and Nucleic Acid Content of Marine Bacterial Communities

dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
dc.contributor.authorHuete-Stauffer, Tamara M.
dc.contributor.authorArandia-Gorostidi, Nestor
dc.contributor.authorAlonso-Sáez, Laura
dc.contributor.authorMoran, Xose Anxelu G.
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.institutionPlankton Ecology and Pelagic Ecosystem Dynamics, Centro Oceanográfico de Gijón/Xixón, Instituto Español de Oceanografía, Gijón/Xixón, Spain
dc.contributor.institutionMarine Research Division, AZTI, Sukarrieta, Spain
dc.date.accessioned2016-06-14T09:18:25Z
dc.date.available2016-06-14T09:18:25Z
dc.date.issued2016-05-23
dc.description.abstractOrganism size reduction with increasing temperature has been suggested as a universal response to global warming. Since genome size is usually correlated to cell size, reduction of genome size in unicells could be a parallel outcome of warming at ecological and evolutionary time scales. In this study, the short-term response of cell size and nucleic acid content of coastal marine prokaryotic communities to temperature was studied over a full annual cycle at a NE Atlantic temperate site. We used flow cytometry and experimental warming incubations, spanning a 6°C range, to analyze the hypothesized reduction with temperature in the size of the widespread flow cytometric bacterial groups of high and low nucleic acid content (HNA and LNA bacteria, respectively). Our results showed decreases in size in response to experimental warming, which were more marked in 0.8 μm pre-filtered treatment rather than in the whole community treatment, thus excluding the role of protistan grazers in our findings. Interestingly, a significant effect of temperature on reducing the average nucleic acid content (NAC) of prokaryotic cells in the communities was also observed. Cell size and nucleic acid decrease with temperature were correlated, showing a common mean decrease of 0.4% per °C. The usually larger HNA bacteria consistently showed a greater reduction in cell and NAC compared with their LNA counterparts, especially during the spring phytoplankton bloom period associated to maximum bacterial growth rates in response to nutrient availability. Our results show that the already smallest planktonic microbes, yet with key roles in global biogeochemical cycling, are likely undergoing important structural shrinkage in response to rising temperatures.
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) through funding of the COMITE (Coastal Ocean MIcrobial plankton and TEmperature) project (CTM-2010-15840) and the associated Ph.D. scholarship (BES-2011-048573) to TH-S, as well as the time-series project RADIALES of the IEO. As well, the Basque Government provided NA-G with a Ph.D. scholarship.
dc.eprint.versionPublisher's Version/PDF
dc.identifier.citationExperimental Warming Decreases the Average Size and Nucleic Acid Content of Marine Bacterial Communities 2016, 7 Frontiers in Microbiology
dc.identifier.doi10.3389/fmicb.2016.00730
dc.identifier.issn1664-302X
dc.identifier.journalFrontiers in Microbiology
dc.identifier.pmid27242747
dc.identifier.urihttp://hdl.handle.net/10754/613034
dc.language.isoen
dc.publisherFrontiers Media SA
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.00730
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. http://creativecommons.org/licenses/by/4.0/
dc.subjectmarine bacteria
dc.subjectcoastal ocean
dc.subjectflow cytometry
dc.subjectsize
dc.subjectnucleic acids
dc.subjecttemperature-size rule
dc.titleExperimental Warming Decreases the Average Size and Nucleic Acid Content of Marine Bacterial Communities
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Huete-Stauffer, Tamara M.,equals">Huete-Stauffer, Tamara M.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Arandia-Gorostidi, Nestor,equals">Arandia-Gorostidi, Nestor</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Alonso-Sáez, Laura,equals">Alonso-Sáez, Laura</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-9823-5339&spc.sf=dc.date.issued&spc.sd=DESC">Moran, Xose Anxelu G.</a> <a href="https://orcid.org/0000-0002-9823-5339" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Sciences and Engineering (BESE) Division,equals">Biological and Environmental Sciences and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Marine Science Program,equals">Marine Science Program</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Red Sea Research Center (RSRC),equals">Red Sea Research Center (RSRC)</a><br><br><h5>Date</h5>2016-05-23</span>
display.details.right<span><h5>Abstract</h5>Organism size reduction with increasing temperature has been suggested as a universal response to global warming. Since genome size is usually correlated to cell size, reduction of genome size in unicells could be a parallel outcome of warming at ecological and evolutionary time scales. In this study, the short-term response of cell size and nucleic acid content of coastal marine prokaryotic communities to temperature was studied over a full annual cycle at a NE Atlantic temperate site. We used flow cytometry and experimental warming incubations, spanning a 6°C range, to analyze the hypothesized reduction with temperature in the size of the widespread flow cytometric bacterial groups of high and low nucleic acid content (HNA and LNA bacteria, respectively). Our results showed decreases in size in response to experimental warming, which were more marked in 0.8 μm pre-filtered treatment rather than in the whole community treatment, thus excluding the role of protistan grazers in our findings. Interestingly, a significant effect of temperature on reducing the average nucleic acid content (NAC) of prokaryotic cells in the communities was also observed. Cell size and nucleic acid decrease with temperature were correlated, showing a common mean decrease of 0.4% per °C. The usually larger HNA bacteria consistently showed a greater reduction in cell and NAC compared with their LNA counterparts, especially during the spring phytoplankton bloom period associated to maximum bacterial growth rates in response to nutrient availability. Our results show that the already smallest planktonic microbes, yet with key roles in global biogeochemical cycling, are likely undergoing important structural shrinkage in response to rising temperatures.<br><br><h5>Citation</h5>Experimental Warming Decreases the Average Size and Nucleic Acid Content of Marine Bacterial Communities 2016, 7 Frontiers in Microbiology<br><br><h5>Acknowledgements</h5>This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) through funding of the COMITE (Coastal Ocean MIcrobial plankton and TEmperature) project (CTM-2010-15840) and the associated Ph.D. scholarship (BES-2011-048573) to TH-S, as well as the time-series project RADIALES of the IEO. As well, the Basque Government provided NA-G with a Ph.D. scholarship.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Frontiers Media SA,equals">Frontiers Media SA</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Frontiers in Microbiology,equals">Frontiers in Microbiology</a><br><br><h5>DOI</h5><a href="https://doi.org/10.3389/fmicb.2016.00730">10.3389/fmicb.2016.00730</a><br><br><h5>PubMed ID</h5><a href="https://www.ncbi.nlm.nih.gov/pubmed/27242747">27242747</a><br><br><h5>Additional Links</h5>http://journal.frontiersin.org/article/10.3389/fmicb.2016.00730</span>
kaust.personMoran, Xose Anxelu G.
orcid.authorHuete-Stauffer, Tamara M.
orcid.authorArandia-Gorostidi, Nestor
orcid.authorAlonso-Sáez, Laura
orcid.authorMoran, Xose Anxelu G.::0000-0002-9823-5339
orcid.id0000-0002-9823-5339
refterms.dateFOA2018-06-13T10:36:35Z
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