Activated and shorter Laser-Scribed graphene Electrodes: Excellent electrochemical signal amplification for detecting biomarkers

dc.contributor.authorFerreira, Daísy Camargo
dc.contributor.authorShetty, Saptami
dc.contributor.authorRizalputri, Lavita
dc.contributor.authorMani, Veerappan
dc.contributor.authorSalama, Khaled N.
dc.contributor.departmentBioengineering
dc.contributor.departmentBioengineering Program
dc.contributor.departmentBiological, Environmental Sciences and Engineering
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering
dc.contributor.departmentComputer, Electrical and Mathematical Science and Engineering (CEMSE) Division
dc.contributor.departmentElectrical and Computer Engineering
dc.contributor.departmentElectrical and Computer Engineering Program
dc.contributor.departmentAdvanced Membranes and Porous Materials Center
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Center
dc.date.accessioned2024-04-01T06:17:20Z
dc.date.available2024-04-01T06:17:20Z
dc.date.issued2024-04
dc.description.abstractLaser-scribed graphene electrodes (LSGEs) are emerging as powerful transducers in electroanalysis. Surface structure and electrode geometry are the two key properties that heavily influence the characteristics of the resulting biosensors. In this work, we have investigated the sensing abilities of the LSGEs at various electrochemical activation procedures and electrode geometry conditions. We found that electrochemically activated LSGEs with shorter electrode connection lengths outperform corresponding non-activated LSGEs with longer electrode connection lengths. The effects of different pH conditions, supporting electrolytes, polarization potentials, and activation time were studied. X-ray photoelectron spectroscopy, Raman spectroscopy, and voltammetry techniques were used to examine the in-situ formation of porosity, introduction of surface oxygen functionalities, role of defect densities, and electrochemically accessible area. Dopamine is used as a model to study the sensing capabilities of the electrodes. Activated LSGE offered a 5.4-fold enhanced detection limit for dopamine compared to longer and non-activated LSGE. Practicality of the method is validated in human serum and urine samples. In addition, the sensor was demonstrated in monitoring in-situ dopamine released by neuroblastoma SH-SY5Y cells. Additionally, the enhanced sensing performance of the activated LSGEs are also tested by sensing uric acid and paracetamol. Electrochemically activated, shorter LSGEs hold great potential in various electrochemical applications.
dc.description.sponsorshipThis research was funded by King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia, and KAUST Smart Health Initiative.
dc.eprint.versionPost-print
dc.identifier.doi10.1016/j.microc.2024.110220
dc.identifier.eid2-s2.0-85188207007
dc.identifier.issn0026-265X
dc.identifier.journalMicrochemical Journal
dc.identifier.pages110220
dc.identifier.urihttps://repository.kaust.edu.sa/handle/10754/697825
dc.identifier.volume199
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0026265X24003321
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microchemical Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microchemical Journal, [199, , (2024-04)] DOI: 10.1016/j.microc.2024.110220 . © 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.embargodate2026-04-01
dc.titleActivated and shorter Laser-Scribed graphene Electrodes: Excellent electrochemical signal amplification for detecting biomarkers
dc.typeArticle
display.details.left<span><h5>Embargo End Date</h5>2026-04-01<br><br><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Ferreira, Daísy Camargo,equals">Ferreira, Daísy Camargo</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-8905-4504&spc.sf=dc.date.issued&spc.sd=DESC">Shetty, Saptami</a> <a href="https://orcid.org/0000-0001-8905-4504" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-6035-6242&spc.sf=dc.date.issued&spc.sd=DESC">Rizalputri, Lavita</a> <a href="https://orcid.org/0000-0001-6035-6242" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-0756-7398&spc.sf=dc.date.issued&spc.sd=DESC">Mani, Veerappan</a> <a href="https://orcid.org/0000-0002-0756-7398" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-7742-1282&spc.sf=dc.date.issued&spc.sd=DESC">Salama, Khaled N.</a> <a href="https://orcid.org/0000-0001-7742-1282" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Bioengineering,equals">Bioengineering</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Bioengineering Program,equals">Bioengineering Program</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological, Environmental Sciences and Engineering,equals">Biological, Environmental Sciences and Engineering</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Science and Engineering (BESE) Division,equals">Biological and Environmental Science and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Computer, Electrical and Mathematical Sciences and Engineering,equals">Computer, Electrical and Mathematical Sciences and Engineering</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division,equals">Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Electrical and Computer Engineering,equals">Electrical and Computer Engineering</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Electrical and Computer Engineering Program,equals">Electrical and Computer Engineering Program</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Advanced Membranes and Porous Materials Center,equals">Advanced Membranes and Porous Materials Center</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Advanced Membranes and Porous Materials Research Center,equals">Advanced Membranes and Porous Materials Research Center</a><br><br><h5>Date</h5>2024-04</span>
display.details.right<span><h5>Abstract</h5>Laser-scribed graphene electrodes (LSGEs) are emerging as powerful transducers in electroanalysis. Surface structure and electrode geometry are the two key properties that heavily influence the characteristics of the resulting biosensors. In this work, we have investigated the sensing abilities of the LSGEs at various electrochemical activation procedures and electrode geometry conditions. We found that electrochemically activated LSGEs with shorter electrode connection lengths outperform corresponding non-activated LSGEs with longer electrode connection lengths. The effects of different pH conditions, supporting electrolytes, polarization potentials, and activation time were studied. X-ray photoelectron spectroscopy, Raman spectroscopy, and voltammetry techniques were used to examine the in-situ formation of porosity, introduction of surface oxygen functionalities, role of defect densities, and electrochemically accessible area. Dopamine is used as a model to study the sensing capabilities of the electrodes. Activated LSGE offered a 5.4-fold enhanced detection limit for dopamine compared to longer and non-activated LSGE. Practicality of the method is validated in human serum and urine samples. In addition, the sensor was demonstrated in monitoring in-situ dopamine released by neuroblastoma SH-SY5Y cells. Additionally, the enhanced sensing performance of the activated LSGEs are also tested by sensing uric acid and paracetamol. Electrochemically activated, shorter LSGEs hold great potential in various electrochemical applications.<br><br><h5>Acknowledgements</h5>This research was funded by King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia, and KAUST Smart Health Initiative.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Elsevier BV,equals">Elsevier BV</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Microchemical Journal,equals">Microchemical Journal</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1016/j.microc.2024.110220">10.1016/j.microc.2024.110220</a><br><br><h5>Additional Links</h5>https://linkinghub.elsevier.com/retrieve/pii/S0026265X24003321</span>
kaust.acknowledged.supportUnitSmart Health Initiative
kaust.personFerreira, Daísy Camargo
kaust.personShetty, Saptami
kaust.personRizalputri, Lavita
kaust.personMani, Veerappan
kaust.personSalama, Khaled N.
orcid.authorFerreira, Daísy Camargo
orcid.authorShetty, Saptami::0000-0001-8905-4504
orcid.authorRizalputri, Lavita::0000-0001-6035-6242
orcid.authorMani, Veerappan::0000-0002-0756-7398
orcid.authorSalama, Khaled N.::0000-0001-7742-1282
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