Assessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis

dc.contributor.authorSharma, Sweta K.
dc.contributor.authorPamidimarri, D. V N N Sudheer
dc.contributor.authorVijay Anand, K. G.
dc.contributor.authorReddy, Muppala P.
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt and Marine Chemical Research Institute (CSIR), G-B Marg., Bhavnagar 364002, India
dc.date.accessioned2015-08-03T09:05:05Z
dc.date.available2015-08-03T09:05:05Z
dc.date.issued2011-07
dc.description.abstractJatropha curcas (Euphorbiaceae), a drought resistant non edible oil yielding plant, has acquired significant importance as an alternative renewable energy source. Low and inconsistent yields found in field plantations prompted for identification of high yielding clones and their large scale multiplication by vegetative propagation to obtain true to type plants. In the current investigation plantlets of J. curcas generated by axillary bud proliferation (micropropagation) using nodal segments obtained from selected high yielding genotypes were assessed for their genetic stability using Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) analyses. For RAPD analysis, 21 out of 52 arbitrary decamer primers screened gave clear reproducible bands. In the micropropagated plantlets obtained from the 2nd sub-culture, 4 out of a total of 177 bands scored were polymorphic, but in the 8th and 16th sub-cultures (culture cycle) no polymorphisms were detected. AFLP analysis revealed 0.63%, 0% and 0% polymorphism in the 2nd, 8th and 16th generations, respectively. When different genotypes, viz. IC 56557 16, IC 56557 34 and IC 56557 13, were assessed by AFLP, 0%, 0.31% and 0.47% polymorphisms were found, respectively, indicating a difference in genetic stability among the different genotypes. To the best of our knowledge this is the first report on assessment of genetic stability of micropropagated plantlets in J. curcas and suggests that axillary shoot proliferation can safely be used as an efficient micropropagation method for mass propagation of J. curcas. © 2011 Elsevier B.V.
dc.description.sponsorshipWe are grateful to Dr. P.K. Ghosh Director, CSMCRI, for his encouragement and Council of Scientific and Industrial Research (CSIR) New Delhi, India for financial support to pursue this work.
dc.identifier.citationSharma, S., Sudheer Pamidimarri, D. V. N., Vijay Anand, K. G., & Reddy, M. P. (2011). Assessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis. Industrial Crops and Products, 34(1), 1003–1009. doi:10.1016/j.indcrop.2011.03.008
dc.identifier.doi10.1016/j.indcrop.2011.03.008
dc.identifier.issn09266690
dc.identifier.journalIndustrial Crops and Products
dc.identifier.urihttp://hdl.handle.net/10754/561808
dc.publisherElsevier BV
dc.subjectAFLP
dc.subjectGenetic stability
dc.subjectJatropha curcas
dc.subjectMicropropagation
dc.subjectRAPD
dc.subjectSomaclonal variations
dc.titleAssessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Sharma, Sweta K.,equals">Sharma, Sweta K.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Pamidimarri, D. V N N Sudheer,equals">Pamidimarri, D. V N N Sudheer</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Vijay Anand, K. G.,equals">Vijay Anand, K. G.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Reddy, Muppala P.,equals">Reddy, Muppala P.</a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Science and Engineering (BESE) Division,equals">Biological and Environmental Science and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Center for Desert Agriculture,equals">Center for Desert Agriculture</a><br><br><h5>Date</h5>2011-07</span>
display.details.right<span><h5>Abstract</h5>Jatropha curcas (Euphorbiaceae), a drought resistant non edible oil yielding plant, has acquired significant importance as an alternative renewable energy source. Low and inconsistent yields found in field plantations prompted for identification of high yielding clones and their large scale multiplication by vegetative propagation to obtain true to type plants. In the current investigation plantlets of J. curcas generated by axillary bud proliferation (micropropagation) using nodal segments obtained from selected high yielding genotypes were assessed for their genetic stability using Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) analyses. For RAPD analysis, 21 out of 52 arbitrary decamer primers screened gave clear reproducible bands. In the micropropagated plantlets obtained from the 2nd sub-culture, 4 out of a total of 177 bands scored were polymorphic, but in the 8th and 16th sub-cultures (culture cycle) no polymorphisms were detected. AFLP analysis revealed 0.63%, 0% and 0% polymorphism in the 2nd, 8th and 16th generations, respectively. When different genotypes, viz. IC 56557 16, IC 56557 34 and IC 56557 13, were assessed by AFLP, 0%, 0.31% and 0.47% polymorphisms were found, respectively, indicating a difference in genetic stability among the different genotypes. To the best of our knowledge this is the first report on assessment of genetic stability of micropropagated plantlets in J. curcas and suggests that axillary shoot proliferation can safely be used as an efficient micropropagation method for mass propagation of J. curcas. © 2011 Elsevier B.V.<br><br><h5>Citation</h5>Sharma, S., Sudheer Pamidimarri, D. V. N., Vijay Anand, K. G., & Reddy, M. P. (2011). Assessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis. Industrial Crops and Products, 34(1), 1003–1009. doi:10.1016/j.indcrop.2011.03.008<br><br><h5>Acknowledgements</h5>We are grateful to Dr. P.K. Ghosh Director, CSMCRI, for his encouragement and Council of Scientific and Industrial Research (CSIR) New Delhi, India for financial support to pursue this work.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Elsevier BV,equals">Elsevier BV</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Industrial Crops and Products,equals">Industrial Crops and Products</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1016/j.indcrop.2011.03.008">10.1016/j.indcrop.2011.03.008</a></span>
kaust.personReddy, Muppala P.
orcid.authorSharma, Sweta K.
orcid.authorPamidimarri, D. V N N Sudheer
orcid.authorVijay Anand, K. G.
orcid.authorReddy, Muppala P.
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