Protocol: MosTI single-copy transgene insertion in C. elegans using heat-shock inducible Cas9 expression

Abstract
Precise expression of transgenes in C. elegans can be used to understand gene regulation, control cells, or as a starting point for genetic screens. Insertion of single-copy transgenes into well-defined safe-harbor locations is useful when a consistent expression levels are required (e.g., to compare expression from different transgenes) or when expression is desired in germ cells. Here, we describe a detailed protocol for inserting single-copy transgenes using Modular Safe-harbor Transgene Insertion (MosTI) and a heat-shock inducible Cas9 expressed from a co-injected plasmid. Inducible Cas9 expression has the advantage of requiring few injections, and many independent single-copy insertions can be generated from a single array line.

Citation
El Mouridi, S., & Frøkjær-Jensen, C. (2022). Protocol: MosTI single-copy transgene insertion in C. elegans using heat-shock inducible Cas9 expression. KAUST Research Repository. https://doi.org/10.25781/KAUST-7ULL8

Acknowledgements
KAUST Office of Sponsored Research (CRG2020-4388)

DOI
10.25781/KAUST-7ULL810.21203/rs.3.rs-2053151/v1

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