Role of the plant-specific endoplasmic reticulum stress-inducible gene TIN1 in the formation of pollen surface structure in Arabidopsis thaliana

Abstract
Accumulation of unfolded proteins in the endoplasmic reticulum (ER) of eukaryotic cells triggers the transcriptional activation of ER-resident molecular chaperones and folding enzymes to maintain cellular homeostasis. This process is known as the ER stress response or the unfolded protein response. We have identified tunicamycin induced 1 (TIN1), a plant-specific ER stress-inducible Arabidopsis thaliana gene. The TIN1 protein is localized in the ER; however, its molecular function has yet to be clarified. In this study, we performed functional analysis of TIN1 in planta. RT-PCR analysis showed that TIN1 is highly expressed in pollen. Analysis using the β-glucuronidase reporter gene demonstrated that the TIN1 promoter is active throughout pollen development, peaking at the time of flowering and in an ovule of an open flower. Although a T-DNA insertion mutant of TIN1 grows normally under ambient laboratory conditions, abnormal pollen surface morphology was observed under a scanning electron microscope. Based on the current and previous observations, a possible physiological function of TIN1 during pollen development is discussed. © 2012 The Japanese Society for Plant Cell and Molecular Biology.

Citation
Iwata, Y., Nishino, T., Iwano, M., Takayama, S., & Koizumi, N. (2012). Role of the plant-specific endoplasmic reticulum stress-inducible gene TIN1 in the formation of pollen surface structure in Arabidopsis thaliana. Plant Biotechnology, 29(1), 51–56. doi:10.5511/plantbiotechnology.11.1228a

Acknowledgements
We would like to thank GABI-Kat and TAIR for the T-DNA insertion mutant and the gene annotation data, respectively. This work was supported by Ministry of Education, Culture, Sports, Science, and Technology of Japan, Grant-in-Aid for Scientific Research 20380188 to N.K.

Publisher
Japanese Society for Plant Cell and Molecular Biology

Journal
Plant Biotechnology

DOI
10.5511/plantbiotechnology.11.1228a

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