Laser-scribed graphene (LSG) as new electrode material for impedance-based cellular assays

dc.contributor.authorPuetz, P.
dc.contributor.authorBehrent, A.
dc.contributor.authorBaeumner, Antje J.
dc.contributor.authorWegener, J.
dc.contributor.institutionInstitute for Analytical Chemistry, Chemo- and Biosensors, University of Regensburg
dc.contributor.institutionFraunhofer Research Institution for Microsystems and Solid State Technologies EMFT, 80686 Muenchen (G)
dc.date.accessioned2021-02-11T12:52:55Z
dc.date.available2021-02-11T12:52:55Z
dc.date.issued2020-10
dc.description.abstractImpedance-based monitoring of cell-based assays has evolved to a multi-functional tool in fundamental and applied biomedical research. The majority of studies rely on gold-films as the electrode material which serves as growth surface and electrode at a time. Besides all its favorable properties like inertness, biocompatibility and superior electrochemical characteristics, gold-film electrodes in contact to cell culture medium show a capacitance of the electrode/electrolyte interface that may become limiting for the sensitivity of the readout. This study describes the use of laser-scribed graphene (LSG) as an alternative electrode material in impedance-based cell monitoring. LSG electrodes are prepared from commercial polyimide foils by simple CO2-laser-induced carbonization. The resulting electrodes show a 25times larger interface capacitance than standard gold-film electrodes due to their foam-like surface topography. Furthermore, LSG-electrodes are (i) highly compatible with cell attachment, (ii) easy to prepare in customized geometries from μm to cm with tunable surface topography and (iii) accessible by roll-to-roll production lines. We conclude from time- and frequency dependent impedance measurements of cell-covered LSG electrodes in direct comparison to the reference material gold that LSG provides an enormous potential to improve the sensitivity of impedance-based monitoring and electric-field mediated manipulation of adherent cells.
dc.description.sponsorshipThis project was funded in part by the Sensor Initiative of the King Abdullah University of Science and Technology (Thuwal, SA) as well as by the Fraunhofer-Gesellschaft (Munich, G) within their funding program Discover. PP is supported by the Studienstiftung des Deutschen Volkes (G).
dc.eprint.versionPost-print
dc.identifier.citationPuetz, P., Behrent, A., Baeumner, A. J., & Wegener, J. (2020). Laser-scribed graphene (LSG) as new electrode material for impedance-based cellular assays. Sensors and Actuators B: Chemical, 321, 128443. doi:10.1016/j.snb.2020.128443
dc.identifier.doi10.1016/j.snb.2020.128443
dc.identifier.eid2-s2.0-85086733146
dc.identifier.issn0925-4005
dc.identifier.journalSensors and Actuators B: Chemical
dc.identifier.pages128443
dc.identifier.urihttp://hdl.handle.net/10754/667363
dc.identifier.volume321
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0925400520307887
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, [321, , (2020-10)] DOI: 10.1016/j.snb.2020.128443 . © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.embargodate2022-06-24
dc.titleLaser-scribed graphene (LSG) as new electrode material for impedance-based cellular assays
dc.typeArticle
display.details.left<span><h5>Embargo End Date</h5>2022-06-24<br><br><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Puetz, P.,equals">Puetz, P.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Behrent, A.,equals">Behrent, A.</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-7148-3423&spc.sf=dc.date.issued&spc.sd=DESC">Baeumner, Antje J.</a> <a href="https://orcid.org/0000-0001-7148-3423" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-8554-0150&spc.sf=dc.date.issued&spc.sd=DESC">Wegener, J.</a> <a href="https://orcid.org/0000-0001-8554-0150" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><br><h5>Date</h5>2020-10</span>
display.details.right<span><h5>Abstract</h5>Impedance-based monitoring of cell-based assays has evolved to a multi-functional tool in fundamental and applied biomedical research. The majority of studies rely on gold-films as the electrode material which serves as growth surface and electrode at a time. Besides all its favorable properties like inertness, biocompatibility and superior electrochemical characteristics, gold-film electrodes in contact to cell culture medium show a capacitance of the electrode/electrolyte interface that may become limiting for the sensitivity of the readout. This study describes the use of laser-scribed graphene (LSG) as an alternative electrode material in impedance-based cell monitoring. LSG electrodes are prepared from commercial polyimide foils by simple CO2-laser-induced carbonization. The resulting electrodes show a 25times larger interface capacitance than standard gold-film electrodes due to their foam-like surface topography. Furthermore, LSG-electrodes are (i) highly compatible with cell attachment, (ii) easy to prepare in customized geometries from μm to cm with tunable surface topography and (iii) accessible by roll-to-roll production lines. We conclude from time- and frequency dependent impedance measurements of cell-covered LSG electrodes in direct comparison to the reference material gold that LSG provides an enormous potential to improve the sensitivity of impedance-based monitoring and electric-field mediated manipulation of adherent cells.<br><br><h5>Citation</h5>Puetz, P., Behrent, A., Baeumner, A. J., & Wegener, J. (2020). Laser-scribed graphene (LSG) as new electrode material for impedance-based cellular assays. Sensors and Actuators B: Chemical, 321, 128443. doi:10.1016/j.snb.2020.128443<br><br><h5>Acknowledgements</h5>This project was funded in part by the Sensor Initiative of the King Abdullah University of Science and Technology (Thuwal, SA) as well as by the Fraunhofer-Gesellschaft (Munich, G) within their funding program Discover. PP is supported by the Studienstiftung des Deutschen Volkes (G).<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Elsevier BV,equals">Elsevier BV</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Sensors and Actuators B: Chemical,equals">Sensors and Actuators B: Chemical</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1016/j.snb.2020.128443">10.1016/j.snb.2020.128443</a><br><br><h5>Additional Links</h5>https://linkinghub.elsevier.com/retrieve/pii/S0925400520307887</span>
kaust.acknowledged.supportUnitSensor Initiative
orcid.authorPuetz, P.
orcid.authorBehrent, A.
orcid.authorBaeumner, Antje J.::0000-0001-7148-3423
orcid.authorWegener, J.::0000-0001-8554-0150
orcid.id0000-0001-8554-0150
orcid.id0000-0001-7148-3423
Files