Supplementary material from "Molecular basis of parental contributions to the behavioural tolerance of elevated pCO in a coral reef fish"

Abstract
Knowledge of adaptive potential is crucial to predicting the impacts of ocean acidification (OA) on marine organisms. In the spiny damselfish, , individual variation in behavioural tolerance to elevated pCO has been observed and is associated with offspring gene expression patterns in the brain. However, the maternal and paternal contributions of this variation are unknown. To investigate parental influence of behavioural pCO tolerance, we crossed pCO-tolerant fathers with pCO-sensitive mothers and vice versa, reared their offspring at control and elevated pCO levels, and compared the juveniles' brain transcriptional program. We identified a large influence of parental phenotype on expression patterns of offspring, irrespective of environmental conditions. Circadian rhythm genes, associated with a tolerant parental phenotype, were uniquely expressed in tolerant mother offspring, while tolerant fathers had a greater role in expression of genes associated with histone binding. Expression changes in genes associated with neural plasticity were identified in both offspring types: the maternal line having a greater effect on genes related to neuron growth while paternal influence impacted the expression of synaptic development genes. Our results confirm cellular mechanisms involved in responses to varying lengths of OA exposure, while highlighting the parental phenotype's influence on offspring molecular phenotype.

Citation
Monroe, A. A., Schunter, C., Welch, M. J., Munday, P. L., & Ravasi, T. (2021). Supplementary material from "Molecular basis of parental contributions to the behavioural tolerance of elevated pCO2 in a coral reef fish". The Royal Society. https://doi.org/10.6084/M9.FIGSHARE.C.5726083

Publisher
The Royal Society

DOI
10.6084/m9.figshare.c.5726083

Additional Links
https://rs.figshare.com/collections/Supplementary_material_from_Molecular_basis_of_parental_contributions_to_the_behavioural_tolerance_of_elevated_pCO_sub_2_sub_in_a_coral_reef_fish_/5726083

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  • [Article]
    Monroe, A. A., Schunter, C., Welch, M. J., Munday, P. L., & Ravasi, T. (2021). Molecular basis of parental contributions to the behavioural tolerance of elevated pCO 2 in a coral reef fish. Proceedings of the Royal Society B: Biological Sciences, 288(1964). doi:10.1098/rspb.2021.1931. DOI: 10.1098/rspb.2021.1931 Handle: 10754/673961

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