Metabolomic Study on Tridacna maxima Giant Clams Reveals Metabolic Fingerprint of Environmental Pollutants

dc.contributor.authorAlmulhim, Fatimah F.
dc.contributor.authorRossbach, Susann
dc.contributor.authorEmwas, Abdul-Hamid M.
dc.contributor.authorKharbatia, Najeh M.
dc.contributor.authorJaremko, Lukasz
dc.contributor.authorJaremko, Mariusz
dc.contributor.authorDuarte, Carlos M.
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentCoreLabs, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
dc.contributor.departmentEnvironmental Lab Organics & Inorganics
dc.contributor.departmentMarine Science Program
dc.contributor.departmentNMR
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.contributor.institutionThe Red Sea Development Company, Department Environmental Sustainability, Riyadh, Saudi Arabia
dc.date.accepted2022-03-15
dc.date.accessioned2022-03-15T13:27:00Z
dc.date.available2022-03-15T13:27:00Z
dc.date.issued2022-04-22
dc.date.submitted2021-11-11
dc.description.abstractMetabolite profiling of marine invertebrates, such as bivalve mollusks, may not only provide insights into the health state of an individual holobiont, but also the pollution levels of their habitats. Here, we combined 1H nuclear magnetic responance (NMR) spectroscopy and mass spectrometry (MS)-based metabolomics techniques to investigate the tissue-specific metabolomic profiles of Tridacna maxima. Animals were collected from across across-shelf gradient in the Red Sea, from inshore to off-shore. We unequivocally profiled 306 metabolites and observed that the collection location had minimal effects on metabolite composition. However, we observed significant differences in metabolite profiles among different tissues (i.e., gills, mantle tissue, and digestive system). Importantly, in addition to endogenous metabolites, we detected the presence of terephthalic acid and isophthalic acid, which likely originate from marine plastic ingestion. Collectively, our study opens opportunities for a deeper understanding of Tridacna maxima physiology through metabolomics, and illustrates the power of invertebrate metabolite profiling for monitoring plastic-related aquatic pollutants.
dc.description.sponsorshipAuthors want to acknowledge the funding support from the KAUST Smart Health Initiative (SHI) seed grants (LJ and MJ) and baseline funds (LJ, MJ, and CD)
dc.eprint.versionPublisher's Version/PDF
dc.identifier.citationAlmulhim, F., Rossbach, S., Emwas, A.-H., Kharbatia, N. M., Jaremko, L., Jaremko, M., & Duarte, C. M. (2022). Metabolomic Study on Tridacna maxima Giant Clams Reveals Metabolic Fingerprint of Environmental Pollutants. Frontiers in Marine Science, 9. https://doi.org/10.3389/fmars.2022.813404
dc.identifier.doi10.3389/fmars.2022.813404
dc.identifier.issn2296-7745
dc.identifier.journalAccepted for publication in Frontiers in Marine Science
dc.identifier.urihttp://hdl.handle.net/10754/675840
dc.identifier.volume9
dc.publisherFrontiers
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fmars.2022.813404/abstract
dc.rightsArchived with thanks to Accepted for publication in Frontiers in Marine Science under a Creative Commons license, details at: https://creativecommons.org/licenses/by/4.0/
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleMetabolomic Study on Tridacna maxima Giant Clams Reveals Metabolic Fingerprint of Environmental Pollutants
dc.typeArticle
display.details.left<span><h5>License</h5>https://creativecommons.org/licenses/by/4.0/<br><br><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-8823-9056&spc.sf=dc.date.issued&spc.sd=DESC">Almulhim, Fatimah F.</a> <a href="https://orcid.org/0000-0001-8823-9056" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Rossbach, Susann,equals">Rossbach, Susann</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Emwas, Abdul-Hamid M.,equals">Emwas, Abdul-Hamid M.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Kharbatia, Najeh M.,equals">Kharbatia, Najeh M.</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-7684-9359&spc.sf=dc.date.issued&spc.sd=DESC">Jaremko, Lukasz</a> <a href="https://orcid.org/0000-0001-7684-9359" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-0787-702X&spc.sf=dc.date.issued&spc.sd=DESC">Jaremko, Mariusz</a> <a href="https://orcid.org/0000-0002-0787-702X" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-1213-1361&spc.sf=dc.date.issued&spc.sd=DESC">Duarte, Carlos M.</a> <a href="https://orcid.org/0000-0002-1213-1361" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Science and Engineering (BESE) Division,equals">Biological and Environmental Science and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Bioscience,equals">Bioscience</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Bioscience Program,equals">Bioscience Program</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Computational Bioscience Research Center (CBRC),equals">Computational Bioscience Research Center (CBRC)</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=CoreLabs, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia,equals">CoreLabs, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Environmental Lab Organics & Inorganics,equals">Environmental Lab Organics & Inorganics</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Marine Science Program,equals">Marine Science Program</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=NMR,equals">NMR</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Red Sea Research Center (RSRC),equals">Red Sea Research Center (RSRC)</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Water Desalination and Reuse Research Center (WDRC),equals">Water Desalination and Reuse Research Center (WDRC)</a><br><br><h5>Date</h5>2022-04-22<br><br><h5>Submitted Date</h5>2021-11-11</span>
display.details.right<span><h5>Abstract</h5>Metabolite profiling of marine invertebrates, such as bivalve mollusks, may not only provide insights into the health state of an individual holobiont, but also the pollution levels of their habitats. Here, we combined 1H nuclear magnetic responance (NMR) spectroscopy and mass spectrometry (MS)-based metabolomics techniques to investigate the tissue-specific metabolomic profiles of Tridacna maxima. Animals were collected from across across-shelf gradient in the Red Sea, from inshore to off-shore. We unequivocally profiled 306 metabolites and observed that the collection location had minimal effects on metabolite composition. However, we observed significant differences in metabolite profiles among different tissues (i.e., gills, mantle tissue, and digestive system). Importantly, in addition to endogenous metabolites, we detected the presence of terephthalic acid and isophthalic acid, which likely originate from marine plastic ingestion. Collectively, our study opens opportunities for a deeper understanding of Tridacna maxima physiology through metabolomics, and illustrates the power of invertebrate metabolite profiling for monitoring plastic-related aquatic pollutants.<br><br><h5>Citation</h5>Almulhim, F., Rossbach, S., Emwas, A.-H., Kharbatia, N. M., Jaremko, L., Jaremko, M., & Duarte, C. M. (2022). Metabolomic Study on Tridacna maxima Giant Clams Reveals Metabolic Fingerprint of Environmental Pollutants. Frontiers in Marine Science, 9. https://doi.org/10.3389/fmars.2022.813404<br><br><h5>Acknowledgements</h5>Authors want to acknowledge the funding support from the KAUST Smart Health Initiative (SHI) seed grants (LJ and MJ) and baseline funds (LJ, MJ, and CD)<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Frontiers,equals">Frontiers</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Accepted for publication in Frontiers in Marine Science,equals">Accepted for publication in Frontiers in Marine Science</a><br><br><h5>DOI</h5><a href="https://doi.org/10.3389/fmars.2022.813404">10.3389/fmars.2022.813404</a><br><br><h5>Additional Links</h5>https://www.frontiersin.org/articles/10.3389/fmars.2022.813404/abstract</span>
kaust.acknowledged.supportUnitSmart Health Initiative
kaust.acknowledged.supportUnitBaseline funds
kaust.personAlmulhim, Fatimah
kaust.personRossbach, Susann
kaust.personEmwas, Abdul-Hamid M.
kaust.personKharbatia, Najeh M.
kaust.personJaremko, Lukasz
kaust.personJaremko, Mariusz
kaust.personDuarte, Carlos M.
orcid.authorAlmulhim, Fatimah F.::0000-0001-8823-9056
orcid.authorRossbach, Susann
orcid.authorEmwas, Abdul-Hamid M.
orcid.authorKharbatia, Najeh M.
orcid.authorJaremko, Lukasz::0000-0001-7684-9359
orcid.authorJaremko, Mariusz::0000-0002-0787-702X
orcid.authorDuarte, Carlos M.::0000-0002-1213-1361
orcid.id0000-0002-1213-1361
orcid.id0000-0002-0787-702X
orcid.id0000-0001-7684-9359
orcid.id0000-0001-8823-9056
refterms.dateFOA2022-04-24T12:30:52Z
Files
Original bundle
Now showing 1 - 1 of 1
Name:
fmars-09-813404 (3).pdf
Size:
3.23 MB
Format:
Adobe Portable Document Format
Description:
Published Version