Increased Antibacterial and Antibiofilm Properties of Silver Nanoparticles Using Silver Fluoride as Precursor

dc.contributor.authorBertoglio, Federico
dc.contributor.authorDe Vita, Lorenzo
dc.contributor.authorD’Agostino, Agnese
dc.contributor.authorDiaz Fernandez, Yuri
dc.contributor.authorFalqui, Andrea
dc.contributor.authorCasu, Alberto
dc.contributor.authorMerli, Daniele
dc.contributor.authorMilanese, Chiara
dc.contributor.authorRossi, Silvia
dc.contributor.authorTaglietti, Angelo
dc.contributor.authorVisai, Livia
dc.contributor.authorPallavicini, Piersandro
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.institutionDipartimento di Medicina Molecolare, Centro per le Tecnologie della Salute (Center for Health Technologies – CHT), UdR INSTM, Università di Pavia, viale Taramelli 3/b, 27100 Pavia, Italy.
dc.contributor.institutionScuola Universitaria Superiore IUSS Pavia, Palazzo del Broletto-Piazza della Vittoria 15, 27100 Pavia, Italy.
dc.contributor.institutionDipartimento di Chimica, Università di Pavia, viale Taramelli 12, 27100 Pavia, Italy.
dc.contributor.institutionDepartment of Chemistry, University of Liverpool, Liverpool L69 3BX, UK.
dc.contributor.institutionDipartimento di Scienze del Farmaco, Università di Pavia, viale Taramelli 12, 27100 Pavia, Italy.
dc.contributor.institutionDipartimento di Medicina Occupazionale, Tossicologia e Rischi Ambientali, Istituti Clinici Scientifici Maugeri S.p.A Società Benefit, IRCCS, Via S. Boezio 28, 27100 Pavia, Italy.
dc.date.accepted2020-07-29
dc.date.accessioned2020-08-10T11:44:08Z
dc.date.available2020-08-10T11:44:08Z
dc.date.issued2020-07-31
dc.date.submitted2020-06-22
dc.description.abstractSilver nanoparticles were produced with AgF as the starting Ag(I) salt, with pectin as the reductant and protecting agent. While the obtained nanoparticles (pAgNP-F) have the same dimensional and physicochemical properties as those already described by us and obtained from AgNO3 and pectin (pAgNP-N), the silver nanoparticles from AgF display an increased antibacterial activity against E. coli PHL628 and Staphylococcus epidermidis RP62A (S. epidermidis RP62A), both as planktonic strains and as their biofilms with respect to pAgNP-N. In particular, a comparison of the antimicrobial and antibiofilm action of pAgNP-F has been carried out with pAgNP-N, pAgNP-N and added NaF, pure AgNO3, pure AgF, AgNO3 and added NaF and pure NaNO3 and NaF salts. By also measuring the concentration of the Ag+ cation released by pAgNP-F and pAgNP-N, we were able to unravel the separate contributions of each potential antibacterial agent, observing an evident synergy between p-AgNP and the F− anion: the F− anion increases the antibacterial power of the p-AgNP solutions even when F− is just 10 µM, a concentration at which F− alone (i.e., as its Na+ salt) is completely ineffective.
dc.description.sponsorshipLDV scholarship was funded by MIUR; this research was also supported by a grant of the Italian Ministry of Education, University and Research (MIUR) to the Department of Molecular Medicine of the University of Pavia under the initiative “Dipartimenti di Eccellenza (2018–2022)”.
dc.eprint.versionPublisher's Version/PDF
dc.identifier.citationBertoglio, F., De Vita, L., D’Agostino, A., Diaz Fernandez, Y., Falqui, A., Casu, A., … Pallavicini, P. (2020). Increased Antibacterial and Antibiofilm Properties of Silver Nanoparticles Using Silver Fluoride as Precursor. Molecules, 25(15), 3494. doi:10.3390/molecules25153494
dc.identifier.doi10.3390/molecules25153494
dc.identifier.issn1420-3049
dc.identifier.issue15
dc.identifier.journalMolecules
dc.identifier.pages3494
dc.identifier.pmid32751978
dc.identifier.urihttp://hdl.handle.net/10754/664538
dc.identifier.volume25
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/1420-3049/25/15/3494
dc.relation.urlhttps://www.mdpi.com/1420-3049/25/15/3494/pdf
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleIncreased Antibacterial and Antibiofilm Properties of Silver Nanoparticles Using Silver Fluoride as Precursor
dc.typeArticle
display.details.left<span><h5>License</h5>https://creativecommons.org/licenses/by/4.0/<br><br><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-6477-1785&spc.sf=dc.date.issued&spc.sd=DESC">Bertoglio, Federico</a> <a href="https://orcid.org/0000-0001-6477-1785" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-8805-6102&spc.sf=dc.date.issued&spc.sd=DESC">De Vita, Lorenzo</a> <a href="https://orcid.org/0000-0001-8805-6102" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=D’Agostino, Agnese,equals">D’Agostino, Agnese</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Diaz Fernandez, Yuri,equals">Diaz Fernandez, Yuri</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-1476-7742&spc.sf=dc.date.issued&spc.sd=DESC">Falqui, Andrea</a> <a href="https://orcid.org/0000-0002-1476-7742" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-5056-2170&spc.sf=dc.date.issued&spc.sd=DESC">Casu, Alberto</a> <a href="https://orcid.org/0000-0002-5056-2170" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Merli, Daniele,equals">Merli, Daniele</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-3763-6657&spc.sf=dc.date.issued&spc.sd=DESC">Milanese, Chiara</a> <a href="https://orcid.org/0000-0002-3763-6657" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-9511-3857&spc.sf=dc.date.issued&spc.sd=DESC">Rossi, Silvia</a> <a href="https://orcid.org/0000-0001-9511-3857" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0003-0141-9201&spc.sf=dc.date.issued&spc.sd=DESC">Taglietti, Angelo</a> <a href="https://orcid.org/0000-0003-0141-9201" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0003-1181-3632&spc.sf=dc.date.issued&spc.sd=DESC">Visai, Livia</a> <a href="https://orcid.org/0000-0003-1181-3632" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-5223-0233&spc.sf=dc.date.issued&spc.sd=DESC">Pallavicini, Piersandro</a> <a href="https://orcid.org/0000-0002-5223-0233" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Sciences and Engineering (BESE) Division,equals">Biological and Environmental Sciences and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Bioscience Program,equals">Bioscience Program</a><br><br><h5>Date</h5>2020-07-31<br><br><h5>Submitted Date</h5>2020-06-22</span>
display.details.right<span><h5>Abstract</h5>Silver nanoparticles were produced with AgF as the starting Ag(I) salt, with pectin as the reductant and protecting agent. While the obtained nanoparticles (pAgNP-F) have the same dimensional and physicochemical properties as those already described by us and obtained from AgNO3 and pectin (pAgNP-N), the silver nanoparticles from AgF display an increased antibacterial activity against E. coli PHL628 and Staphylococcus epidermidis RP62A (S. epidermidis RP62A), both as planktonic strains and as their biofilms with respect to pAgNP-N. In particular, a comparison of the antimicrobial and antibiofilm action of pAgNP-F has been carried out with pAgNP-N, pAgNP-N and added NaF, pure AgNO3, pure AgF, AgNO3 and added NaF and pure NaNO3 and NaF salts. By also measuring the concentration of the Ag+ cation released by pAgNP-F and pAgNP-N, we were able to unravel the separate contributions of each potential antibacterial agent, observing an evident synergy between p-AgNP and the F− anion: the F− anion increases the antibacterial power of the p-AgNP solutions even when F− is just 10 µM, a concentration at which F− alone (i.e., as its Na+ salt) is completely ineffective.<br><br><h5>Citation</h5>Bertoglio, F., De Vita, L., D’Agostino, A., Diaz Fernandez, Y., Falqui, A., Casu, A., … Pallavicini, P. (2020). Increased Antibacterial and Antibiofilm Properties of Silver Nanoparticles Using Silver Fluoride as Precursor. Molecules, 25(15), 3494. doi:10.3390/molecules25153494<br><br><h5>Acknowledgements</h5>LDV scholarship was funded by MIUR; this research was also supported by a grant of the Italian Ministry of Education, University and Research (MIUR) to the Department of Molecular Medicine of the University of Pavia under the initiative “Dipartimenti di Eccellenza (2018–2022)”.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=MDPI AG,equals">MDPI AG</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Molecules,equals">Molecules</a><br><br><h5>DOI</h5><a href="https://doi.org/10.3390/molecules25153494">10.3390/molecules25153494</a><br><br><h5>PubMed ID</h5><a href="https://www.ncbi.nlm.nih.gov/pubmed/32751978">32751978</a><br><br><h5>Additional Links</h5>https://www.mdpi.com/1420-3049/25/15/3494https://www.mdpi.com/1420-3049/25/15/3494/pdf</span>
kaust.personFalqui, Andrea
kaust.personCasu, Alberto
orcid.authorBertoglio, Federico::0000-0001-6477-1785
orcid.authorDe Vita, Lorenzo::0000-0001-8805-6102
orcid.authorD’Agostino, Agnese
orcid.authorDiaz Fernandez, Yuri
orcid.authorFalqui, Andrea::0000-0002-1476-7742
orcid.authorCasu, Alberto::0000-0002-5056-2170
orcid.authorMerli, Daniele
orcid.authorMilanese, Chiara::0000-0002-3763-6657
orcid.authorRossi, Silvia::0000-0001-9511-3857
orcid.authorTaglietti, Angelo::0000-0003-0141-9201
orcid.authorVisai, Livia::0000-0003-1181-3632
orcid.authorPallavicini, Piersandro::0000-0002-5223-0233
orcid.id0000-0002-5223-0233
orcid.id0000-0003-1181-3632
orcid.id0000-0003-0141-9201
orcid.id0000-0001-9511-3857
orcid.id0000-0002-3763-6657
orcid.id0000-0002-5056-2170
orcid.id0000-0002-1476-7742
orcid.id0000-0001-8805-6102
orcid.id0000-0001-6477-1785
refterms.dateFOA2020-08-10T11:44:53Z
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