Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans

dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
dc.contributor.authorTeira, Eva
dc.contributor.authorHernando-Morales, Víctor
dc.contributor.authorCornejo-Castillo, Francisco M.
dc.contributor.authorAlonso-Sáez, Laura
dc.contributor.authorSarmento, Hugo
dc.contributor.authorValencia-Vila, Joaquín
dc.contributor.authorSerrano Catalá, Teresa
dc.contributor.authorHernández-Ruiz, Marta
dc.contributor.authorVarela, Marta M.
dc.contributor.authorFerrera, Isabel
dc.contributor.authorMoran, Xose Anxelu G.
dc.contributor.authorGasol, Josep M.
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.institutionUniversidade de Vigo, Vigo, Spain
dc.contributor.institutionInstitut de Ciències del Mar, CSIC, Barcelona, Spain
dc.contributor.institutionAZTI, Sukarrieta, Spain
dc.contributor.institutionDepartment of Hydrobiology, Federal University of São Carlos, São Carlos, São Paulo, Brazil
dc.contributor.institutionInstituto Español de Oceanografía, Centro Oceanográfico de A Coruña, A Coruña, Spain
dc.contributor.institutionUniversidad de Granada, Granada, Spain
dc.contributor.institutionInstituto Español de Oceanografía, Centro Oceanográfico de Xixón, Xixón, Spain
dc.date.accessioned2015-11-22T12:09:02Z
dc.date.available2015-11-22T12:09:02Z
dc.date.issued2015-09-25
dc.date.published-online2015-09-25
dc.date.published-print2015-12-01
dc.description.abstractThe transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the world's oceans, where the growth of the natural prokaryotic assemblages was promoted by filtration (i.e., removal of grazers [F treatment]) or filtration combined with dilution (i.e., also relieving resource competition [FD treatment]). The impact of sunlight exposure was also evaluated in the FD treatments, and we did not find a significant effect on the eCFs. The eCFs varied from 0.09 to 1.47 kg C mol Leu−1 and were significantly lower in the FD than in the F samples. Also, changes in bacterial community composition during the incubations, as assessed by automated ribosomal intergenic spacer analysis (ARISA), were more pronounced in the FD than in the F treatments, compared to unmanipulated controls. Thus, we discourage the common procedure of diluting samples (in addition to filtration) for eCF determination. The eCFs in the filtered treatment were negatively correlated with the initial chlorophyll a concentration, picocyanobacterial abundance (mostly Prochlorococcus), and the percentage of heterotrophic prokaryotes with high nucleic acid content (%HNA). The latter two variables explained 80% of the eCF variability in the F treatment, supporting the view that both Prochlorococcus and HNA prokaryotes incorporate leucine in substantial amounts, although this results in relatively low carbon production rates in the oligotrophic ocean.
dc.eprint.versionPublisher's Version/PDF
dc.identifier.citationSample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans 2015, 81 (23):8224 Applied and Environmental Microbiology
dc.identifier.doi10.1128/AEM.02454-15
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.journalApplied and Environmental Microbiology
dc.identifier.pmid26407885
dc.identifier.urihttp://hdl.handle.net/10754/582473
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.relation.urlhttp://aem.asm.org/lookup/doi/10.1128/AEM.02454-15
dc.rightsArchived with thanks to Applied and Environmental Microbiology
dc.titleSample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Teira, Eva,equals">Teira, Eva</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Hernando-Morales, Víctor,equals">Hernando-Morales, Víctor</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Cornejo-Castillo, Francisco M.,equals">Cornejo-Castillo, Francisco M.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Alonso-Sáez, Laura,equals">Alonso-Sáez, Laura</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Sarmento, Hugo,equals">Sarmento, Hugo</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Valencia-Vila, Joaquín,equals">Valencia-Vila, Joaquín</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Serrano Catalá, Teresa,equals">Serrano Catalá, Teresa</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Hernández-Ruiz, Marta,equals">Hernández-Ruiz, Marta</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Varela, Marta M.,equals">Varela, Marta M.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Ferrera, Isabel,equals">Ferrera, Isabel</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-9823-5339&spc.sf=dc.date.issued&spc.sd=DESC">Moran, Xose Anxelu G.</a> <a href="https://orcid.org/0000-0002-9823-5339" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Gasol, Josep M.,equals">Gasol, Josep M.</a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Red Sea Research Center (RSRC),equals">Red Sea Research Center (RSRC)</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Sciences and Engineering (BESE) Division,equals">Biological and Environmental Sciences and Engineering (BESE) Division</a><br><br><h5>Online Publication Date</h5>2015-09-25<br><br><h5>Print Publication Date</h5>2015-12-01<br><br><h5>Date</h5>2015-09-25</span>
display.details.right<span><h5>Abstract</h5>The transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the world's oceans, where the growth of the natural prokaryotic assemblages was promoted by filtration (i.e., removal of grazers [F treatment]) or filtration combined with dilution (i.e., also relieving resource competition [FD treatment]). The impact of sunlight exposure was also evaluated in the FD treatments, and we did not find a significant effect on the eCFs. The eCFs varied from 0.09 to 1.47 kg C mol Leu−1 and were significantly lower in the FD than in the F samples. Also, changes in bacterial community composition during the incubations, as assessed by automated ribosomal intergenic spacer analysis (ARISA), were more pronounced in the FD than in the F treatments, compared to unmanipulated controls. Thus, we discourage the common procedure of diluting samples (in addition to filtration) for eCF determination. The eCFs in the filtered treatment were negatively correlated with the initial chlorophyll a concentration, picocyanobacterial abundance (mostly Prochlorococcus), and the percentage of heterotrophic prokaryotes with high nucleic acid content (%HNA). The latter two variables explained 80% of the eCF variability in the F treatment, supporting the view that both Prochlorococcus and HNA prokaryotes incorporate leucine in substantial amounts, although this results in relatively low carbon production rates in the oligotrophic ocean.<br><br><h5>Citation</h5>Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans 2015, 81 (23):8224 Applied and Environmental Microbiology<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=American Society for Microbiology,equals">American Society for Microbiology</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Applied and Environmental Microbiology,equals">Applied and Environmental Microbiology</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1128/AEM.02454-15">10.1128/AEM.02454-15</a><br><br><h5>PubMed ID</h5><a href="https://www.ncbi.nlm.nih.gov/pubmed/26407885">26407885</a><br><br><h5>Additional Links</h5>http://aem.asm.org/lookup/doi/10.1128/AEM.02454-15</span>
orcid.authorTeira, Eva
orcid.authorHernando-Morales, Víctor
orcid.authorCornejo-Castillo, Francisco M.
orcid.authorAlonso-Sáez, Laura
orcid.authorSarmento, Hugo
orcid.authorValencia-Vila, Joaquín
orcid.authorSerrano Catalá, Teresa
orcid.authorHernández-Ruiz, Marta
orcid.authorVarela, Marta M.
orcid.authorFerrera, Isabel
orcid.authorMoran, Xose Anxelu G.::0000-0002-9823-5339
orcid.authorGasol, Josep M.
orcid.id0000-0002-9823-5339
refterms.dateFOA2018-06-13T11:34:27Z
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