Genetic diversity and biogeographical patterns of Caulerpa prolifera across the Mediterranean and Mediterranean/Atlantic transition zone

dc.contributor.authorVarela-Álvarez, Elena
dc.contributor.authorBalau, Ana C.
dc.contributor.authorMarbà, Núrià N.
dc.contributor.authorAfonso-Carrillo, Julio
dc.contributor.authorDuarte, Carlos M.
dc.contributor.authorSerrão, Ester Álvares
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.institutionCentro de Ciências do Mar, CCMAR-CIMAR, Universidade do Algarve, Campus de GambelasFaro, Portugal
dc.contributor.institutionDepartment of Global Change Research, IMEDEA, CSIC-UIB, Instituto Mediterráneo de Estudios AvanzadosEsporles, Mallorca, Spain
dc.contributor.institutionDepartamento de Biología Vegetal (Botánica), Universidad de La LagunaLa Laguna,Canary Islands, Spain
dc.date.accessioned2015-08-03T12:22:43Z
dc.date.available2015-08-03T12:22:43Z
dc.date.issued2015-01-11
dc.date.published-online2015-01-11
dc.date.published-print2015-03
dc.description.abstractKnowledge of spatial patterns of genetic differentiation between populations is key to understanding processes in evolutionary history of biological species. Caulerpa is a genus of marine green algae, which has attracted much public attention, mainly because of the impacts of invasive species in the Mediterranean. However, very little is known about the ecological and evolutionary history of the Mediterranean native Caulerpa prolifera, a species which is currently found at sites distributed worldwide. C. prolifera provides a good model to explore the patterns of genetic diversity at different scales across the Mediterranean and Atlantic area. This study aims to investigate the biogeographical patterns of diversity and differentiation of C. prolifera in the Mediterranean, with special focus on the Mediterranean/Atlantic transition zone. We used two nuclear (ITS rDNA and the hypervariable microsatellite locus CaPr_J2) and one chloroplast (tufA) DNA markers on samples of C. prolifera from its entire range. Analyses of 51 sequences of the cpDNA tufA of C. prolifera, 87 ITS2 sequences and genotypes of 788 ramets of C. prolifera for the locus CaPr_J2 revealed three different biogeographical areas: West Atlantic, East Atlantic and a larger area representing the Mediterranean, the Mediterranean/Atlantic transition zone and a Pacific site (Bali). It was found out that the Mediterranean/Atlantic transition zone is a biogeographical boundary for C. prolifera. A lack of connectivity was revealed between Atlantic and Mediterranean types, and identical sequences found in the Mediterranean and Indo-Pacific suggest either recent gene flow along the Red Sea connection or a possible ancient Indo-Pacific origin.
dc.description.sponsorshipWe thank all the people and Institutions that collected plants and/or helped with the sampling collection: Liam Cronin, Ligia Collado-Vides, Gisela Dionisio, Elvira Alvarez, Rocio Santiago, Elena Diaz-Almela, Ricardo Bermejo, Fernando Ojeda Copete, Alexandra Cunha, Pablo Manent, Estibaliz Berecibar, Antonia Ribera-Siguan, Carolina Pena-Martin, Pablo Sanchez Jerez, Felio Lozano, Jacinto Barquin and Francisco Valdes; Natural Park of s'Albufera des Grau (Government of the Balearic Islands: http://www.balearsnatura.com/parc-natural-de-s-albufera-des-grau/equipamiento.html) and the Marine Reserve of Tabarca (Alicante). We also thank for the sequencing work Marta Valente and Cristina Paulino. Financial support for this study was provided by several research projects: CAULERPA_GENETICS (PTDC/MAR/70921/2006) by FCT (Portuguese Science Foundation) co-funded by FEDER and a postdoctoral fellowship (SFRH/BPD/17206/2004) from FCT, Portugal, co-funded by FSE, both to EVA, EXCL/AAG-GLO/0661/2012 to ES, CAULEXPAN (Spanish Ministry of Education, REN2002-00701) to NM, MedVeg (EU contract no. Q5RS-2001-02456), PRADERAS by the Foundation BBVA (Banco Bilbao Vizcaya Argentaria) to CD.
dc.identifier.citationVarela-Álvarez, E., Balau, A. C., Marbà, N., Afonso-Carrillo, J., Duarte, C. M., & Serrão, E. A. (2015). Genetic diversity and biogeographical patterns of Caulerpa prolifera across the Mediterranean and Mediterranean/Atlantic transition zone. Marine Biology, 162(3), 557–569. doi:10.1007/s00227-014-2605-5
dc.identifier.doi10.1007/s00227-014-2605-5
dc.identifier.issn00253162
dc.identifier.journalMarine Biology
dc.identifier.urihttp://hdl.handle.net/10754/564004
dc.publisherSpringer Nature
dc.titleGenetic diversity and biogeographical patterns of Caulerpa prolifera across the Mediterranean and Mediterranean/Atlantic transition zone
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Varela-Álvarez, Elena,equals">Varela-Álvarez, Elena</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Balau, Ana C.,equals">Balau, Ana C.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Marbà, Núrià N.,equals">Marbà, Núrià N.</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Afonso-Carrillo, Julio,equals">Afonso-Carrillo, Julio</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-1213-1361&spc.sf=dc.date.issued&spc.sd=DESC">Duarte, Carlos M.</a> <a href="https://orcid.org/0000-0002-1213-1361" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Serrão, Ester Álvares,equals">Serrão, Ester Álvares</a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Red Sea Research Center (RSRC),equals">Red Sea Research Center (RSRC)</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Sciences and Engineering (BESE) Division,equals">Biological and Environmental Sciences and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Marine Science Program,equals">Marine Science Program</a><br><br><h5>Online Publication Date</h5>2015-01-11<br><br><h5>Print Publication Date</h5>2015-03<br><br><h5>Date</h5>2015-01-11</span>
display.details.right<span><h5>Abstract</h5>Knowledge of spatial patterns of genetic differentiation between populations is key to understanding processes in evolutionary history of biological species. Caulerpa is a genus of marine green algae, which has attracted much public attention, mainly because of the impacts of invasive species in the Mediterranean. However, very little is known about the ecological and evolutionary history of the Mediterranean native Caulerpa prolifera, a species which is currently found at sites distributed worldwide. C. prolifera provides a good model to explore the patterns of genetic diversity at different scales across the Mediterranean and Atlantic area. This study aims to investigate the biogeographical patterns of diversity and differentiation of C. prolifera in the Mediterranean, with special focus on the Mediterranean/Atlantic transition zone. We used two nuclear (ITS rDNA and the hypervariable microsatellite locus CaPr_J2) and one chloroplast (tufA) DNA markers on samples of C. prolifera from its entire range. Analyses of 51 sequences of the cpDNA tufA of C. prolifera, 87 ITS2 sequences and genotypes of 788 ramets of C. prolifera for the locus CaPr_J2 revealed three different biogeographical areas: West Atlantic, East Atlantic and a larger area representing the Mediterranean, the Mediterranean/Atlantic transition zone and a Pacific site (Bali). It was found out that the Mediterranean/Atlantic transition zone is a biogeographical boundary for C. prolifera. A lack of connectivity was revealed between Atlantic and Mediterranean types, and identical sequences found in the Mediterranean and Indo-Pacific suggest either recent gene flow along the Red Sea connection or a possible ancient Indo-Pacific origin.<br><br><h5>Citation</h5>Varela-Álvarez, E., Balau, A. C., Marbà, N., Afonso-Carrillo, J., Duarte, C. M., & Serrão, E. A. (2015). Genetic diversity and biogeographical patterns of Caulerpa prolifera across the Mediterranean and Mediterranean/Atlantic transition zone. Marine Biology, 162(3), 557–569. doi:10.1007/s00227-014-2605-5<br><br><h5>Acknowledgements</h5>We thank all the people and Institutions that collected plants and/or helped with the sampling collection: Liam Cronin, Ligia Collado-Vides, Gisela Dionisio, Elvira Alvarez, Rocio Santiago, Elena Diaz-Almela, Ricardo Bermejo, Fernando Ojeda Copete, Alexandra Cunha, Pablo Manent, Estibaliz Berecibar, Antonia Ribera-Siguan, Carolina Pena-Martin, Pablo Sanchez Jerez, Felio Lozano, Jacinto Barquin and Francisco Valdes; Natural Park of s'Albufera des Grau (Government of the Balearic Islands: http://www.balearsnatura.com/parc-natural-de-s-albufera-des-grau/equipamiento.html) and the Marine Reserve of Tabarca (Alicante). We also thank for the sequencing work Marta Valente and Cristina Paulino. Financial support for this study was provided by several research projects: CAULERPA_GENETICS (PTDC/MAR/70921/2006) by FCT (Portuguese Science Foundation) co-funded by FEDER and a postdoctoral fellowship (SFRH/BPD/17206/2004) from FCT, Portugal, co-funded by FSE, both to EVA, EXCL/AAG-GLO/0661/2012 to ES, CAULEXPAN (Spanish Ministry of Education, REN2002-00701) to NM, MedVeg (EU contract no. Q5RS-2001-02456), PRADERAS by the Foundation BBVA (Banco Bilbao Vizcaya Argentaria) to CD.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Springer Nature,equals">Springer Nature</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Marine Biology,equals">Marine Biology</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1007/s00227-014-2605-5">10.1007/s00227-014-2605-5</a></span>
kaust.personDuarte, Carlos M.
orcid.authorVarela-Álvarez, Elena
orcid.authorBalau, Ana C.
orcid.authorMarbà, Núrià N.
orcid.authorAfonso-Carrillo, Julio
orcid.authorDuarte, Carlos M.::0000-0002-1213-1361
orcid.authorSerrão, Ester Álvares
orcid.id0000-0002-1213-1361
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