Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)

dc.contributor.authorSugumar, Thennarasu
dc.contributor.authorGanesan, Pugalenthi
dc.contributor.authorHarishankar, Murugesan
dc.contributor.authorDhinakar Raj, Gopal
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentCore Labs
dc.contributor.institutionDepartment of Immunology, Tuberculosis Research Centre, Indian Council of Medical Research, Chennai, India
dc.contributor.institutionDepartment of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India
dc.date.accessioned2015-08-03T11:11:38Z
dc.date.available2015-08-03T11:11:38Z
dc.date.issued2013-06-25
dc.date.published-online2013-06-25
dc.date.published-print2014-02
dc.description.abstractGranulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.
dc.description.sponsorshipThe authors thank the Department of Biotechnology (DBT), Government of India, for the financial assistance to this Project. ST and MHS were SRFs who worked in this project. GP carried out the bioinformatics analysis.
dc.identifier.citationSugumar, T., Pugalenthi, G., Harishankar, M., & Dhinakar Raj, G. (2013). Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis). International Journal of Immunogenetics, 41(1), 74–80. doi:10.1111/iji.12074
dc.identifier.doi10.1111/iji.12074
dc.identifier.issn17443121
dc.identifier.journalInternational Journal of Immunogenetics
dc.identifier.pmid23800159
dc.identifier.urihttp://hdl.handle.net/10754/562823
dc.publisherWiley
dc.titleMolecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Sugumar, Thennarasu,equals">Sugumar, Thennarasu</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Ganesan, Pugalenthi,equals">Ganesan, Pugalenthi</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Harishankar, Murugesan,equals">Harishankar, Murugesan</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Dhinakar Raj, Gopal,equals">Dhinakar Raj, Gopal</a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Bioscience Core Lab,equals">Bioscience Core Lab</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Core Labs,equals">Core Labs</a><br><br><h5>Online Publication Date</h5>2013-06-25<br><br><h5>Print Publication Date</h5>2014-02<br><br><h5>Date</h5>2013-06-25</span>
display.details.right<span><h5>Abstract</h5>Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.<br><br><h5>Citation</h5>Sugumar, T., Pugalenthi, G., Harishankar, M., & Dhinakar Raj, G. (2013). Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis). International Journal of Immunogenetics, 41(1), 74–80. doi:10.1111/iji.12074<br><br><h5>Acknowledgements</h5>The authors thank the Department of Biotechnology (DBT), Government of India, for the financial assistance to this Project. ST and MHS were SRFs who worked in this project. GP carried out the bioinformatics analysis.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Wiley,equals">Wiley</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=International Journal of Immunogenetics,equals">International Journal of Immunogenetics</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1111/iji.12074">10.1111/iji.12074</a><br><br><h5>PubMed ID</h5><a href="https://www.ncbi.nlm.nih.gov/pubmed/23800159">23800159</a></span>
kaust.personSugumar, Thennarasu
kaust.personGanesan, Pugalenthi
orcid.authorSugumar, Thennarasu
orcid.authorGanesan, Pugalenthi
orcid.authorHarishankar, Murugesan
orcid.authorDhinakar Raj, Gopal
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