Subcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers

dc.contributor.authorGinsawaeng, Orarat
dc.contributor.authorHeise, Carolin
dc.contributor.authorSangwan, Rohit
dc.contributor.authorKarcher, Daniel
dc.contributor.authorHernández-Sánchez, Itzell Euridice
dc.contributor.authorSampathkumar, Arun
dc.contributor.authorZuther, Ellen
dc.contributor.institutionMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam, Germany
dc.date.accessioned2021-12-12T13:44:46Z
dc.date.available2021-12-12T13:44:46Z
dc.date.issued2021-11-25
dc.description.abstractLEA proteins are involved in plant stress tolerance. In Arabidopsis, the LEA_4 Pfam group is the biggest group with the majority of its members being expressed in dry seeds. To assess subcellular localization in vivo, we investigated 11 seed-expressed LEA_4 proteins in embryos dissected from dry seeds expressing LEA_4 fusion proteins under its native promoters with the Venus fluorescent protein (proLEA_4
dc.description.sponsorshipWe acknowledge the Max Planck Society for funding. OG was supported by a PhD scholarship of the Royal Thai Government. IEHS gratefully acknowledges funding by a Consejo Nacional de Ciencia y Tecnologia (CONACYT) postdoctoral grant from Mexico (423770).
dc.eprint.versionPublisher's Version/PDF
dc.identifier.citationGinsawaeng, O., Heise, C., Sangwan, R., Karcher, D., Hernández-Sánchez, I. E., Sampathkumar, A., & Zuther, E. (2021). Subcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers. Biomolecules, 11(12), 1770. doi:10.3390/biom11121770
dc.identifier.doi10.3390/biom11121770
dc.identifier.eid2-s2.0-85119692099
dc.identifier.issn2218-273X
dc.identifier.issue12
dc.identifier.journalBiomolecules
dc.identifier.pages1770
dc.identifier.urihttp://hdl.handle.net/10754/673996
dc.identifier.volume11
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2218-273X/11/12/1770
dc.rightsArchived with thanks to Biomolecules
dc.titleSubcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Ginsawaeng, Orarat,equals">Ginsawaeng, Orarat</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Heise, Carolin,equals">Heise, Carolin</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Sangwan, Rohit,equals">Sangwan, Rohit</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Karcher, Daniel,equals">Karcher, Daniel</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Hernández-Sánchez, Itzell Euridice,equals">Hernández-Sánchez, Itzell Euridice</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0003-1703-0137&spc.sf=dc.date.issued&spc.sd=DESC">Sampathkumar, Arun</a> <a href="https://orcid.org/0000-0003-1703-0137" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Zuther, Ellen,equals">Zuther, Ellen</a><br><br><h5>Date</h5>2021-11-25</span>
display.details.right<span><h5>Abstract</h5>LEA proteins are involved in plant stress tolerance. In Arabidopsis, the LEA_4 Pfam group is the biggest group with the majority of its members being expressed in dry seeds. To assess subcellular localization in vivo, we investigated 11 seed-expressed LEA_4 proteins in embryos dissected from dry seeds expressing LEA_4 fusion proteins under its native promoters with the Venus fluorescent protein (proLEA_4<br><br><h5>Citation</h5>Ginsawaeng, O., Heise, C., Sangwan, R., Karcher, D., Hernández-Sánchez, I. E., Sampathkumar, A., & Zuther, E. (2021). Subcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers. Biomolecules, 11(12), 1770. doi:10.3390/biom11121770<br><br><h5>Acknowledgements</h5>We acknowledge the Max Planck Society for funding. OG was supported by a PhD scholarship of the Royal Thai Government. IEHS gratefully acknowledges funding by a Consejo Nacional de Ciencia y Tecnologia (CONACYT) postdoctoral grant from Mexico (423770).<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=MDPI AG,equals">MDPI AG</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Biomolecules,equals">Biomolecules</a><br><br><h5>DOI</h5><a href="https://doi.org/10.3390/biom11121770">10.3390/biom11121770</a><br><br><h5>Additional Links</h5>https://www.mdpi.com/2218-273X/11/12/1770</span>
orcid.authorGinsawaeng, Orarat
orcid.authorHeise, Carolin
orcid.authorSangwan, Rohit
orcid.authorKarcher, Daniel
orcid.authorHernández-Sánchez, Itzell Euridice
orcid.authorSampathkumar, Arun::0000-0003-1703-0137
orcid.authorZuther, Ellen
orcid.id0000-0003-1703-0137
refterms.dateFOA2021-12-12T13:46:34Z
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