Subcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers

Abstract
LEA proteins are involved in plant stress tolerance. In Arabidopsis, the LEA_4 Pfam group is the biggest group with the majority of its members being expressed in dry seeds. To assess subcellular localization in vivo, we investigated 11 seed-expressed LEA_4 proteins in embryos dissected from dry seeds expressing LEA_4 fusion proteins under its native promoters with the Venus fluorescent protein (proLEA_4

Citation
Ginsawaeng, O., Heise, C., Sangwan, R., Karcher, D., Hernández-Sánchez, I. E., Sampathkumar, A., & Zuther, E. (2021). Subcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers. Biomolecules, 11(12), 1770. doi:10.3390/biom11121770

Acknowledgements
We acknowledge the Max Planck Society for funding. OG was supported by a PhD scholarship of the Royal Thai Government. IEHS gratefully acknowledges funding by a Consejo Nacional de Ciencia y Tecnologia (CONACYT) postdoctoral grant from Mexico (423770).

Publisher
MDPI AG

Journal
Biomolecules

DOI
10.3390/biom11121770

Additional Links
https://www.mdpi.com/2218-273X/11/12/1770

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