Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red Sea, Saudi Arabia

Type
Article

Authors
Lai, PokYui
Miao, Li
Lee, Onon
Liu, Lingli
Zhou, Xiaojian
Xü, Ying
Al-Suwailem, Abdulaziz M.
Qian, Pei-Yuan

KAUST Department
Coastal and Marine Resources Core Lab

KAUST Grant Number
SA-C0040/UK-C001

Online Publication Date
2012-06-08

Print Publication Date
2013-03-01

Date
2012-06-08

Abstract
A slow-growing, strictly aerobic, Gram-negative, coccus bacterial strain, designated KAUST100406-0324T, was isolated from sea-floor sediment collected from the Red Sea, Saudi Arabia. The catalase- and oxidase-positive strain was non-sporulating and only slightly halophilic. Optimum growth occurred at 20-25 °C and at pH values ranging from 7.0 to 8.0. The major cellular fatty acids of the strain were unsaturated C18: 1ω6c and/or C18:1ω7c, C18:1ω7c 11-methyl and C16:1ω7c and/or C16:1ω6c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. Ubiquinone 10 was the predominant lipoquinone. The DNA G+C content of strain KAUST100406-0324T was 64.0 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that the novel strain belonged to the family Rhodobacteraceae of the class Alphaproteobacteria but formed a distinct evolutionary lineage from other bacterial species with validly published names. The 16S rRNA gene sequence of the novel strain was distantly related, but formed a monophyletic cluster with, those of bacteria from two moderately halophilic genera, Hwanghaeicola and Maribius. The similarity of the sequence between the novel strain KAUST100406-0324T and the type strains Hwanghaeicola aestuarii Y26T (accession number FJ230842), Maribius pelagius B5-6T (DQ514326) and Maribius salinus CL-SP27T (AY906863) were 94.5 %, 95.2 % and 95.3 %, respectively. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we propose that this strain represents a novel species of a new genus in the family Rhodobacteraceae, for which the name of Profundibacterium mesophilum gen. nov., sp. nov. was proposed, with KAUST100406-0324T (= JCM 17872T = NRRL B-59665T) as the type strain. © 2013 IUMS.

Citation
Lai, P. Y., Miao, L., Lee, O. O., Liu, L.-L., Zhou, X.-J., Xu, Y., … Qian, P.-Y. (2013). Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family

              Rhodobacteraceae
           
         isolated from deep-sea sediment in the Red Sea, Saudi Arabia. International Journal of Systematic and Evolutionary Microbiology, 63(Pt_3), 1007–1012. doi:10.1099/ijs.0.041525-0<br><br><h5>Acknowledgements</h5>The authors thank H. C. Chung and S. Arellano from Hong Kong University of Science and Technology (HKUST) for collecting sediment samples from the Red Sea; the technical team from King Abdullah University of Science and Technology (KAUST) for coordinating the 2010 expedition cruise, and J. P. Euzeby, Immunologie, Ecole Nationale Veterinaire de Toulouse, France, for advice on nomenclature. This publication was based on work supported by KAUST award no. SA-C0040/UK-C001.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Microbiology Society,equals">Microbiology Society</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,equals">INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1099/ijs.0.041525-0">10.1099/ijs.0.041525-0</a><br><br><h5>PubMed ID</h5><a href="https://www.ncbi.nlm.nih.gov/pubmed/22685108">22685108</a></span>
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