Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant

Abstract
We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.

Citation
Priyadarshini, M., AlHarbi, S., & Frøkjær-Jensen, C. (2022). Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant. microPublication Biology. https://doi.org/10.17912/MICROPUB.BIOLOGY.000638

Acknowledgements
KAUST intramural funding and an OSR competitive research grant (CRG10 URF/1/4705-01-01). We thank the CGC, which is funded by the NIH Office of Research Infrastructure Programs (grant no. P40 OD010440) and WormBase for data and literature curation.

Journal
microPublication biology

DOI
10.17912/micropub.biology.000638

PubMed ID
36188099

PubMed Central ID
PMC9520340

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