RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome

dc.contributor.authorKöster, Tino
dc.contributor.authorMarondedze, Claudius
dc.contributor.authorMeyer, Katja
dc.contributor.authorStaiger, Dorothee
dc.contributor.institutionMolecular Cell Physiology, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germany
dc.contributor.institutionCambridge Centre for Proteomics, Cambridge Systems Biology Centre, Cambridge, UK
dc.contributor.institutionDepartment of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
dc.date.accessioned2017-05-15T10:35:10Z
dc.date.available2017-05-15T10:35:10Z
dc.date.issued2017-04-13
dc.date.published-online2017-04-13
dc.date.published-print2017-06
dc.description.abstractRNA–protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture – where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.
dc.description.sponsorshipWe thank Prof. Michael Ladomery for comments on the draft of this manuscript. We gratefully acknowledge support by the Deutsche Forschungsgemeinschaft (DFG) through grants STA653 and SPP1530. C.M. was funded by the Office of Competitive Research Grant Program, grant CRG3-62140383, from King Abdullah University of Science and Technology (KAUST). K.M. was funded by the German National Academic Foundation.
dc.identifier.citationKöster T, Marondedze C, Meyer K, Staiger D (2017) RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome. Trends in Plant Science. Available: http://dx.doi.org/10.1016/j.tplants.2017.03.009.
dc.identifier.doi10.1016/j.tplants.2017.03.009
dc.identifier.issn1360-1385
dc.identifier.journalTrends in Plant Science
dc.identifier.urihttp://hdl.handle.net/10754/623586
dc.publisherElsevier BV
dc.subjectRNA-binding protein
dc.subjectmRNA interactome
dc.subjectUV crosslinking
dc.subjectmRNP particles
dc.titleRNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Köster, Tino,equals">Köster, Tino</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-2113-904X&spc.sf=dc.date.issued&spc.sd=DESC">Marondedze, Claudius</a> <a href="https://orcid.org/0000-0002-2113-904X" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Meyer, Katja,equals">Meyer, Katja</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Staiger, Dorothee,equals">Staiger, Dorothee</a><br><br><h5>KAUST Grant Number</h5>CRG3-62140383<br><br><h5>Online Publication Date</h5>2017-04-13<br><br><h5>Print Publication Date</h5>2017-06<br><br><h5>Date</h5>2017-04-13</span>
display.details.right<span><h5>Abstract</h5>RNA–protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture – where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.<br><br><h5>Citation</h5>Köster T, Marondedze C, Meyer K, Staiger D (2017) RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome. Trends in Plant Science. Available: http://dx.doi.org/10.1016/j.tplants.2017.03.009.<br><br><h5>Acknowledgements</h5>We thank Prof. Michael Ladomery for comments on the draft of this manuscript. We gratefully acknowledge support by the Deutsche Forschungsgemeinschaft (DFG) through grants STA653 and SPP1530. C.M. was funded by the Office of Competitive Research Grant Program, grant CRG3-62140383, from King Abdullah University of Science and Technology (KAUST). K.M. was funded by the German National Academic Foundation.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=Elsevier BV,equals">Elsevier BV</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Trends in Plant Science,equals">Trends in Plant Science</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1016/j.tplants.2017.03.009">10.1016/j.tplants.2017.03.009</a></span>
kaust.grant.numberCRG3-62140383
orcid.authorKöster, Tino
orcid.authorMarondedze, Claudius::0000-0002-2113-904X
orcid.authorMeyer, Katja
orcid.authorStaiger, Dorothee
orcid.id0000-0002-2113-904X
Files