RNA editing differently affects protein-coding genes in D. melanogaster and H. sapiens

Type
Article

Authors
Grassi, Luigi
Leoni, Guido
Tramontano, Anna

KAUST Grant Number
KUK-I1-012-43

Date
2015

Abstract
When an RNA editing event occurs within a coding sequence it can lead to a different encoded amino acid. The biological significance of these events remains an open question: they can modulate protein functionality, increase the complexity of transcriptomes or arise from a loose specificity of the involved enzymes. We analysed the editing events in coding regions that produce or not a change in the encoded amino acid (nonsynonymous and synonymous events, respectively) in D. melanogaster and in H. sapiens and compared them with the appropriate random models. Interestingly, our results show that the phenomenon has rather different characteristics in the two organisms. For example, we confirm the observation that editing events occur more frequently in non-coding than in coding regions, and report that this effect is much more evident in H. sapiens. Additionally, in this latter organism, editing events tend to affect less conserved residues. The less frequently occurring editing events in Drosophila tend to avoid drastic amino acid changes. Interestingly, we find that, in Drosophila, changes from less frequently used codons to more frequently used ones are favoured, while this is not the case in H. sapiens.

Citation
Grassi, L., Leoni, G., & Tramontano, A. (2015). RNA editing differently affects protein-coding genes in D. melanogaster and H. sapiens. Scientific Reports, 5(1). doi:10.1038/srep11550

Acknowledgements
All the authors are very grateful to Marco Cosentino Lagomarsino for useful discussions. The support by KAUST Award KUK-I1-012-43, PRIN 20108XYHJS and the Epigenomics flagship project EPIGEN is gratefully acknowledged.

Publisher
NATURE PORTFOLIO

Journal
SCIENTIFIC REPORTS

DOI
10.1038/srep11550

PubMed ID
26169954

PubMed Central ID
PMC4648400

Additional Links
http://www.nature.com/articles/srep11550

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