Cardiac Progenitor Cell Extraction from Human Auricles

Type
Book Chapter

Authors
Di Nardo, Paolo
Pagliari, Francesca

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) Division
Physical Science and Engineering (PSE) Division

Online Publication Date
2017-02-23

Print Publication Date
2017

Date
2017-02-23

Abstract
For many years, myocardial tissue has been considered terminally differentiated and, thus, incapable of regenerating. Recent studies have shown, instead, that cardiomyocytes, at least in part, are slowly substituted by new cells originating by precursor cells mostly embedded into the heart apex and in the atria. We have shown that an elective region of progenitor cell embedding is represented by the auricles, non-contractile atria appendages that can be easily sampled without harming the patient. The protocol here reported describes how from auricles a population of multipotent, cardiogenic cells can be isolated, cultured, and differentiated. Further studies are needed to fully exploit this cell population, but, sampling auricles, it could be possible to treat cardiac patients using their own cells circumventing rejection or organ shortage limitations.

Citation
Di Nardo P, Pagliari F (2017) Cardiac Progenitor Cell Extraction from Human Auricles. Methods in Molecular Biology: 145–154. Available: http://dx.doi.org/10.1007/978-1-4939-6756-8_11.

Publisher
Springer Nature

Journal
Methods in Molecular Biology

DOI
10.1007/978-1-4939-6756-8_11

Additional Links
http://link.springer.com/chapter/10.1007/978-1-4939-6756-8_11

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