Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

Type
Book Chapter

Authors
Barrios-Llerena, Martin
Le Bihan, Thierry

KAUST Department
Bioscience Core Lab
Proteomics and Protein Expression

Online Publication Date
2019-04-13

Print Publication Date
2019

Date
2019-04-13

Abstract
Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.

Citation
Barrios-Llerena ME, Le Bihan T (2019) Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation. Mass Spectrometry of Proteins: 35–42. Available: http://dx.doi.org/10.1007/978-1-4939-9232-4_4.

Publisher
Springer Nature

Journal
Mass Spectrometry of Proteins

DOI
10.1007/978-1-4939-9232-4_4

Additional Links
https://link.springer.com/protocol/10.1007%2F978-1-4939-9232-4_4

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