Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

Type
Article

Authors
Kogan, Maxim
Beke-Somfai, Tamás
Nordén, Bengt

KAUST Grant Number
KUK-11-008-23

Date
2011

Abstract
Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.

Citation
Kogan M, Beke-Somfai T, Nordén B (2011) Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light. Chem Commun 47: 7356. Available: http://dx.doi.org/10.1039/c1cc12313k.

Acknowledgements
This work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).

Publisher
Royal Society of Chemistry (RSC)

Journal
Chemical Communications

DOI
10.1039/c1cc12313k

PubMed ID
21637893

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