Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

dc.contributor.authorPerozziello, Gerardo
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorDe Grazia, Antonio
dc.contributor.authorEsposito, Francesco
dc.contributor.authorAllione, Marco
dc.contributor.authorColuccio, Maria Laura
dc.contributor.authorTallerico, Rossana
dc.contributor.authorValpapuram, Immanuel
dc.contributor.authorTirinato, Luca
dc.contributor.authorDas, Gobind
dc.contributor.authorGiugni, Andrea
dc.contributor.authorTorre, Bruno
dc.contributor.authorVeltri, Pierangelo
dc.contributor.authorKruhne, Ulrich
dc.contributor.authorDella Valle, Giuseppe
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, BioNEM lab, University Magna Graecia of Catanzaro, Catanzaro, Italy
dc.contributor.institutionDepartment of Surgical and Medical Science, University Magna Graecia of Catanzaro, Catanzaro, Italy
dc.contributor.institutionDepartment of Chemical and Biochemical Engineering, Technical University of Denmark (DTU), Lyngby, Denmark
dc.contributor.institutionDepartment of Physics, Istituto di Fotonica E Nanotecnologie - CNR, Politecnico di Milano, Milano, Italy
dc.date.accessioned2016-11-03T08:29:29Z
dc.date.available2016-11-03T08:29:29Z
dc.date.issued2015-12-11
dc.date.published-online2015-12-11
dc.date.published-print2016-01-25
dc.description.abstractIn this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.
dc.description.sponsorshipThis work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.
dc.eprint.versionPost-print
dc.identifier.citationPerozziello G, Candeloro P, De Grazia A, Esposito F, Allione M, et al. (2015) Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express 24: A180. Available: http://dx.doi.org/10.1364/oe.24.00a180.
dc.identifier.doi10.1364/oe.24.00a180
dc.identifier.issn1094-4087
dc.identifier.journalOptics Express
dc.identifier.urihttp://hdl.handle.net/10754/621445
dc.publisherThe Optical Society
dc.relation.urlhttp://pdfs.semanticscholar.org/2553/e31d581d8b5eac91eac4359fb9fcef692819.pdf
dc.rightsArchived with thanks to The Optical Society
dc.rightsThis file is an open access version redistributed from: http://pdfs.semanticscholar.org/2553/e31d581d8b5eac91eac4359fb9fcef692819.pdf
dc.titleMicrofluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers
dc.typeArticle
display.details.left<span><h5>Type</h5>Article<br><br><h5>Authors</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Perozziello, Gerardo,equals">Perozziello, Gerardo</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-6156-887X&spc.sf=dc.date.issued&spc.sd=DESC">Candeloro, Patrizio</a> <a href="https://orcid.org/0000-0001-6156-887X" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0002-8935-7801&spc.sf=dc.date.issued&spc.sd=DESC">De Grazia, Antonio</a> <a href="https://orcid.org/0000-0002-8935-7801" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Esposito, Francesco,equals">Esposito, Francesco</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0003-0757-9791&spc.sf=dc.date.issued&spc.sd=DESC">Allione, Marco</a> <a href="https://orcid.org/0000-0003-0757-9791" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Coluccio, Maria Laura,equals">Coluccio, Maria Laura</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Tallerico, Rossana,equals">Tallerico, Rossana</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Valpapuram, Immanuel,equals">Valpapuram, Immanuel</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-9826-2129&spc.sf=dc.date.issued&spc.sd=DESC">Tirinato, Luca</a> <a href="https://orcid.org/0000-0001-9826-2129" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0003-0942-681X&spc.sf=dc.date.issued&spc.sd=DESC">Das, Gobind</a> <a href="https://orcid.org/0000-0003-0942-681X" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Giugni, Andrea,equals">Giugni, Andrea</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Torre, Bruno,equals">Torre, Bruno</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Veltri, Pierangelo,equals">Veltri, Pierangelo</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Kruhne, Ulrich,equals">Kruhne, Ulrich</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.author=Della Valle, Giuseppe,equals">Della Valle, Giuseppe</a><br><a href="https://repository.kaust.edu.sa/search?query=orcid.id:0000-0001-5886-4678&spc.sf=dc.date.issued&spc.sd=DESC">Di Fabrizio, Enzo M.</a> <a href="https://orcid.org/0000-0001-5886-4678" target="_blank"><img src="https://repository.kaust.edu.sa/server/api/core/bitstreams/82a625b4-ed4b-40c8-865a-d6a5225a26a4/content" width="16" height="16"/></a><br><br><h5>KAUST Department</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Biological and Environmental Sciences and Engineering (BESE) Division,equals">Biological and Environmental Sciences and Engineering (BESE) Division</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Material Science and Engineering Program,equals">Material Science and Engineering Program</a><br><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.department=Physical Science and Engineering (PSE) Division,equals">Physical Science and Engineering (PSE) Division</a><br><br><h5>Online Publication Date</h5>2015-12-11<br><br><h5>Print Publication Date</h5>2016-01-25<br><br><h5>Date</h5>2015-12-11</span>
display.details.right<span><h5>Abstract</h5>In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.<br><br><h5>Citation</h5>Perozziello G, Candeloro P, De Grazia A, Esposito F, Allione M, et al. (2015) Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express 24: A180. Available: http://dx.doi.org/10.1364/oe.24.00a180.<br><br><h5>Acknowledgements</h5>This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.<br><br><h5>Publisher</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.publisher=The Optical Society,equals">The Optical Society</a><br><br><h5>Journal</h5><a href="https://repository.kaust.edu.sa/search?spc.sf=dc.date.issued&spc.sd=DESC&f.journal=Optics Express,equals">Optics Express</a><br><br><h5>DOI</h5><a href="https://doi.org/10.1364/oe.24.00a180">10.1364/oe.24.00a180</a><br><br><h5>Additional Links</h5>http://pdfs.semanticscholar.org/2553/e31d581d8b5eac91eac4359fb9fcef692819.pdf</span>
kaust.personDe Grazia, Antonio
kaust.personAllione, Marco
kaust.personColuccio, Maria Laura
kaust.personValpapuram, Immanuel
kaust.personTirinato, Luca
kaust.personDas, Gobind
kaust.personGiugni, Andrea
kaust.personTorre, Bruno
kaust.personDi Fabrizio, Enzo M.
orcid.authorPerozziello, Gerardo
orcid.authorCandeloro, Patrizio::0000-0001-6156-887X
orcid.authorDe Grazia, Antonio::0000-0002-8935-7801
orcid.authorEsposito, Francesco
orcid.authorAllione, Marco::0000-0003-0757-9791
orcid.authorColuccio, Maria Laura
orcid.authorTallerico, Rossana
orcid.authorValpapuram, Immanuel
orcid.authorTirinato, Luca::0000-0001-9826-2129
orcid.authorDas, Gobind::0000-0003-0942-681X
orcid.authorGiugni, Andrea
orcid.authorTorre, Bruno
orcid.authorVeltri, Pierangelo
orcid.authorKruhne, Ulrich
orcid.authorDella Valle, Giuseppe
orcid.authorDi Fabrizio, Enzo M.::0000-0001-5886-4678
orcid.id0000-0001-5886-4678
orcid.id0000-0003-0942-681X
orcid.id0000-0001-9826-2129
orcid.id0000-0003-0757-9791
orcid.id0000-0002-8935-7801
orcid.id0000-0001-6156-887X
refterms.dateFOA2021-06-23T06:50:36Z
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