Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Abstract
In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.

Citation
Perozziello G, Candeloro P, De Grazia A, Esposito F, Allione M, et al. (2015) Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers. Optics Express 24: A180. Available: http://dx.doi.org/10.1364/oe.24.00a180.

Acknowledgements
This work was partially supported by the European project EUROMBR (grant no. 608104), Cariplo Foundation under the project "New Frontiers in Plasmonic Nanosensing" (Grant no. 2011-0338), the project for Young researchers financed from the Ministry of Health "High Throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices" (CUP J65C13001350001, project no. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University "Magna Graecia" of Catanzaro.

Publisher
The Optical Society

Journal
Optics Express

DOI
10.1364/oe.24.00a180

Additional Links
http://pdfs.semanticscholar.org/2553/e31d581d8b5eac91eac4359fb9fcef692819.pdf

Permanent link to this record