Early-stage organoid formation in biofunctionalized self-assembling peptide matrices combinations
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MS Thesis
Embargo End Date:
2024-08-24
Type
ThesisAuthors
Xu, Jiayi
Advisors
Hauser, Charlotte
Committee members
Schmidt, Fabian
Al-Sulaiman, Dana

Program
BioengineeringKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionDate
2023-08-14Embargo End Date
2024-08-24Permanent link to this record
http://hdl.handle.net/10754/693751
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At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis will become available to the public after the expiration of the embargo on 2024-08-24.Abstract
As the third most commonly diagnosed cancer in the world, colorectal cancer (CRC) has become a pressing and urgent problem, requiring the disease mechanism research and therapeutic development. The field of tissue engineering has considerably progressed since the advent of synthetic matrices for 3D cell culture, providing in vitro models for CRC disease research. Compared to animal-derived matrices such as matrigel, synthetic matrices have several advantages including controllable properties, avoiding ethical problems and batch-to-batch variation. Ultrashort self-assembly peptides of amphiphilic nature have recently proven to be excellent matrices for 3D cell culture of many types of cells. In this thesis, we aimed to use biofunctional peptides to promote the growth of colorectal cancer organoids in the early stage of development. A peptide-based biofunctional hydrogel for organoid culture has been developed for the purpose of establishing a reproducible colorectal cancer model. The hydrogel is composed of self-assembly peptides designed to induce cell-matrix interactions. The hydrogel is mechanically tunable with customizable cell-adhesive ligands and has the ability to promote the formation and growth of colorectal cancer organoids in vitro. One of these biofunctionalized peptide matrices was particularly suitable for CRC lumen development, apical protein expression, and cell differentiation level compared to the gold-standard ECM Matrigel.ae974a485f413a2113503eed53cd6c53
10.25781/KAUST-96842