Water-ultrastable perovskite CsPbBr3 nanocrystals for fluorescence-enhanced cellular imaging
AuthorsHe, Cai Li
Meng, Zi Qi
Ren, Shu Xia
Hao, Wei Zhong
Chen, Shu Lin
Yan, Rong Rong
Zhu, Yu Fang
Zhao, Jin Jin
Embargo End Date2024-02-28
Permanent link to this recordhttp://hdl.handle.net/10754/690059
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AbstractMetal halide perovskites have attracted much attention in biomedicine because of their excellent fluorescence energy conversion properties; however, poor water-stability and cytotoxicity limit its applications as a biomedical tracer, especially in cellular imaging. Herein, water-ultrastable perovskites CsPbBr3:Cs4PbBr6 nanocrystals (NCs) encapsulated in chitosan are fabricated successfully using a water-triggered method. The as-synthesized CsPbBr3:Cs4PbBr6@CS (chitosan, CS) nanoparticles in water display enhanced fluorescence emission for 35 days. Further, the viability of glioma cells (U87 cells) incubated with different concentrations of CsPbBr3:Cs4PbBr6@CS nanoparticles (0–20 μg·ml−1) for 24 h is found to be higher than 90%. In artificial body fluid, analyses using laser confocal microscopy, the standard Cell Counting Kit-8 (CCK-8) method, and flow cytometry demonstrated the good water ultrastability and high biocompatibility performance of CsPbBr3:Cs4PbBr6@CS nanoparticles in cellular imaging. Overall, the water-ultrastable halide perovskites support promising perspectives in biological cell tracing and intelligent medical technology.
CitationHe, C.-L., Meng, Z.-Q., Ren, S.-X., Li, J., Wang, Y., Wu, H., Bu, H., Zhang, Y., Hao, W.-Z., Chen, S.-L., Yan, R.-R., Liu, H., Zhu, Y.-F., & Zhao, J.-J. (2023). Water-ultrastable perovskite CsPbBr3 nanocrystals for fluorescence-enhanced cellular imaging. Rare Metals. https://doi.org/10.1007/s12598-022-02222-8
SponsorsThis work was financially supported by the National Natural Science Foundation of China (Nos. U2130128 and 11772207), the Natural Science Foundation of Hebei Province (Nos. A2019210204 and H2022205047), the Central Government Guiding Local Science and Technology Development Project (No. 216Z4302G), the Natural Science Foundation of Hebei Education Department (No. ZD2020192), the Youth Top-notch Talents Supporting Plan of Hebei Province, National Key Research and Development Program of China (No. 2016YFC0904503) and Hebei Administration for Market Supervision Science and Technology Project List (2023ZC03). The authors gratefully acknowledge the Electron Microscopy Laboratory at Peking University for the use of electron microscopes.
PublisherSpringer Science and Business Media LLC