Photostable polymorphic organic cages for targeted live cell imaging
AuthorsAl Kelabi, Dana
Alimi, Lukman Olawale
Piwonski, Hubert Marek
Khashab, Niveen M.
KAUST DepartmentSmart Hybrid Materials (SHMs) Laboratory, Advanced Membranes and Porous Materials Center, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
Biological and Environmental Science and Engineering (BESE) Division
Advanced Membranes and Porous Materials Research Center
Physical Science and Engineering (PSE) Division
Chemical Science Program
Permanent link to this recordhttp://hdl.handle.net/10754/679126
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AbstractFluorescent microscopy is a powerful tool for studying the cellular dynamics of biological systems. Small-molecule organic fluorophores are the most commonly used for live cell imaging; however, they often suffer from low solubility, limited photostability and variable targetability. Herein, we demonstrate that a tautomeric organic cage, OC1, has high cell permeability, photostability and selectivity towards the mitochondria. We further performed a structure–activity study to investigate the role of the keto–enol tautomerization, which affords strong and consistent fluorescence in dilute solutions through supramolecular self-assembly. Significantly, OC1 can passively diffuse through the cell membrane directly targeting the mitochondria without going through the endosomes or the lysosomes. We envisage that designing highly stable and biocompatible self-assembled fluorophores that can passively diffuse through the cell membrane while selectively targeting specific organelles will push the boundaries of fluorescent microscopy to visualize intricate cellular processes at the single molecule level in live samples.
CitationAl Kelabi, D., Dey, A., Alimi, L. O., Piwoński, H., Habuchi, S., & Khashab, N. M. (2022). Photostable polymorphic organic cages for targeted live cell imaging. Chemical Science. https://doi.org/10.1039/d2sc00836j
PublisherRoyal Society of Chemistry (RSC)
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