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dc.contributor.authorMoretti, Manola
dc.contributor.authorLimongi, Tania
dc.contributor.authorTesti, Claudia
dc.contributor.authorMilanetti, Edoardo
dc.contributor.authorDe Angelis, Maria Teresa
dc.contributor.authorParrotta, Elvira I
dc.contributor.authorScalise, Stefania
dc.contributor.authorSantamaria, Gianluca
dc.contributor.authorAllione, Marco
dc.contributor.authorLopatin, Sergei
dc.contributor.authorTorre, Bruno
dc.contributor.authorZhang, Peng
dc.contributor.authorMarini, Monica
dc.contributor.authorPerozziello, Gerardo
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorPirri, Candido Fabrizio
dc.contributor.authorRuocco, Giancarlo
dc.contributor.authorCuda, Giovanni
dc.contributor.authorDi Fabrizio, Enzo
dc.date.accessioned2022-05-22T11:38:42Z
dc.date.available2022-05-22T11:38:42Z
dc.date.issued2022-05-20
dc.identifier.citationMoretti, M., Limongi, T., Testi, C., Milanetti, E., De Angelis, M. T., Parrotta, E. I., Scalise, S., Santamaria, G., Allione, M., Lopatin, S., Torre, B., Zhang, P., Marini, M., Perozziello, G., Candeloro, P., Pirri, C. F., Ruocco, G., Cuda, G., & Di Fabrizio, E. (2022). Direct Visualization and Identification of Membrane Voltage-Gated Sodium Channels from Human iPSC-Derived Neurons by Multiple Imaging and Light Enhanced Spectroscopy. Small Methods, 2200402. Portico. https://doi.org/10.1002/smtd.202200402
dc.identifier.issn2366-9608
dc.identifier.pmid35595684
dc.identifier.doi10.1002/smtd.202200402
dc.identifier.doi10.1002/smtd.202270040
dc.identifier.urihttp://hdl.handle.net/10754/678098
dc.description.abstractIn this study, transmission electron microscopy atomic force microscopy, and surface enhanced Raman spectroscopy are combined through a direct imaging approach, to gather structural and chemical information of complex molecular systems such as ion channels in their original plasma membrane. Customized microfabricated sample holder allows to characterize Nav channels embedded in the original plasma membrane extracted from neuronal cells that are derived from healthy human induced pluripotent stem cells. The identification of the channels is accomplished by using two different approaches, one of them widely used in cryo-EM (the particle analysis method) and the other based on a novel Zernike Polynomial expansion of the images bitmap. This approach allows to carry out a whole series of investigations, one complementary to the other, on the same sample, preserving its state as close as possible to the original membrane configuration.
dc.description.sponsorshipThe authors acknowledge financial support from King Abdullah University of Science and Technology for OCRF-2014-CRG and OCRF-2016-CRG grants; SHAHEEN cluster to provide machine time; Professor Charlotte A. E. Hauser for providing access to SHAHEEN, and from Piedmont Region through European Funds for Regional Development (“Food Digital Monitoring” project); European Research Council Synergy grant ASTRA (n. 855923).
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.1002/smtd.202200402
dc.rights© 2022 The Authors. Small Methods published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribu-tion-NonCommercial-NoDerivs License, which permits use and distribu-tion in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAFM
dc.subjectRaman spectroscopy
dc.subjectTEM
dc.subjectSuperhydrophobic Surfaces
dc.subjectZernike Polynomial
dc.subjectVoltage Gated Sodium Channels
dc.titleDirect Visualization and Identification of Membrane Voltage-Gated Sodium Channels from Human iPSC-Derived Neurons by Multiple Imaging and Light Enhanced Spectroscopy
dc.typeArticle
dc.contributor.departmentKing Abdullah University of Science and Technology, SMILEs lab, PSE Division, Thuwal, 23955-6900, Kingdom of Saudi Arabia.
dc.contributor.departmentKing Abdullah University of Science and Technology, Imaging and Characterization Core lab, Thuwal, 23955-6900, Kingdom of Saudi Arabia.
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentElectron Microscopy
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.contributor.departmentImaging and Characterization Core Lab
dc.identifier.journalSmall methods
dc.eprint.versionPublisher's Version/PDF
kaust.personMoretti, Manola
kaust.personAllione, Marco
kaust.personLopatin, Sergei
kaust.personTorre, Bruno
kaust.personZhang, Peng
kaust.grant.numberOCRF-2014-CRG
kaust.grant.numberOCRF-2016-CRG
refterms.dateFOA2022-05-22T11:40:21Z
kaust.acknowledged.supportUnitSHAHEEN cluster


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© 2022 The Authors. Small Methods published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribu-tion-NonCommercial-NoDerivs License, which permits use and distribu-tion in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made
Except where otherwise noted, this item's license is described as © 2022 The Authors. Small Methods published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribu-tion-NonCommercial-NoDerivs License, which permits use and distribu-tion in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made