Continuous extraction and concentration of secreted metabolites from engineered microbes using membrane technology
Beke, Aron K.
Lauersen, Kyle J.
KAUST DepartmentMarine Science Program
Biological and Environmental Science and Engineering (BESE) Division
Chemical Engineering Program
Physical Science and Engineering (PSE) Division
Water Desalination and Reuse Research Center (WDRC)
Environmental Science and Engineering Program
Advanced Membranes and Porous Materials Research Center
KAUST Grant Number4238
Permanent link to this recordhttp://hdl.handle.net/10754/677942
MetadataShow full item record
AbstractMicroalgal cultivation in photobioreactors and membrane separations are both considered sustainable processes. Here we explore their synergistic combination to extract and concentrate a heterologous sesquiterpenoid produced by engineered green algal cells. A hydrophobic hollow-fiber membrane contactor was used to allow interaction of culture broth and cells with a dodecane solvent phase to accumulate algal produced patchoulol. Subsequent continuous membrane extraction of patchoulol from dodecane enabled product concentration in a methanol stream as well as dodecane recovery for its reuse. A structure-based prediction using machine learning was used to model a process whereby 100% patchoulol recovery from dodecane could be achieved with solvent-resistant nanofiltration membranes. Solvent consumption, E-factor, and economic sustainability were assessed and compared with existing patchoulol production processes. Our extraction and product purification process offers six- and two-orders of magnitude lower solvent consumption compared to synthetic production and thermal-based separation, respectively. Our proposed methodology is transferable to other microbial systems for the isolation of high-value isoprenoid and hydrocarbon products.
CitationOvermans, S., Ignacz, G., Beke, A. K., Xu, J., Saikaly, P., Szekely, G., & Lauersen, K. J. (2022). Continuous extraction and concentration of secreted metabolites from engineered microbes using membrane technology. Green Chemistry. https://doi.org/10.1039/d2gc00938b
SponsorsWe would like to express special thanks to Dr. Najeh Kharbatia of the KAUST Analytical Core Labs for helpful early discussions, Chandrasekaren Lakshmipathy, Abdulkhalik Khalifa, and Abdullah Alabdullatif of KAUST Lab Equipment Maintenance (LEM) team for assistance in upgrading and initializing the GC-FID-MS unit. The authors acknowledge Prof. Dr. Ralph Bock for providing C. reinhardtii UVM4, obtained under material transfer agreement between KAUST and the Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam. The research reported in this publication was supported by the KAUST Impact Acceleration Funds program (grant 4238), and KAUST baseline funding awarded to KL and GS. Figure 1 was produced by Ana Bigio, scientific illustrator.
PublisherRoyal Society of Chemistry (RSC)
CollectionsArticles; Bioengineering Program; Biological and Environmental Science and Engineering (BESE) Division; Advanced Membranes and Porous Materials Research Center; Environmental Science and Engineering Program; Marine Science Program; Physical Science and Engineering (PSE) Division; Chemical Engineering Program; Water Desalination and Reuse Research Center (WDRC)
Except where otherwise noted, this item's license is described as Archived with thanks to Green Chemistry under a Creative Commons license.