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dc.contributor.authorCapasso, Laura
dc.contributor.authorZoccola, Didier
dc.contributor.authorGanot, Philippe
dc.contributor.authorAranda, Manuel
dc.contributor.authorTambutté, Sylvie
dc.date.accessioned2022-04-14T12:26:29Z
dc.date.available2022-04-14T12:26:29Z
dc.date.issued2022-04-13
dc.identifier.citationCapasso, L., Zoccola, D., Ganot, P., Aranda, M., & Tambutté, S. (2022). SpiAMT1d: molecular characterization, localization, and potential role in coral calcification of an ammonium transporter in Stylophora pistillata. Coral Reefs. https://doi.org/10.1007/s00338-022-02256-5
dc.identifier.issn0722-4028
dc.identifier.issn1432-0975
dc.identifier.doi10.1007/s00338-022-02256-5
dc.identifier.urihttp://hdl.handle.net/10754/676262
dc.description.abstractMembers of the ammonium transporter family occur in all domains of life. However, they have been characterized, at the molecular level, mainly in bacteria and plants, whereas in animals their molecular characterization is limited to a few species. In marine invertebrates, recent studies have reported a multitude of physiological functions in which ammonium could take part. Among them, calcification is one for which very few data are available. In scleractinian corals, it has been reported that external sources of ammonium and/or ammonium containing compounds enhance calcification. However, despite these physiological insights, the underlying transport mechanisms are still unknown at the molecular level. In this study, we performed a first molecular characterization of ammonium transporters in the scleractinian coral Stylophora pistillata. By performing differential gene expression analysis, through quantitative real-time PCR, we show that S. pistillata ammonium transporters possess different tissue specificities. Most notably, one ammonium transporter, spiAMT1d, is specifically expressed in the coral tissue containing the calcifying cells. Furthermore, we determined spiAMT1d subcellular localization by immunostaining S. pistillata histological cross-sections and show that it localizes on the apical side of the calcifying cells and in their intracellular compartments. Taken together our results strongly suggest a role of ammonium transporters in coral calcification and lay the groundwork for many future studies aiming to better elucidate the potential role of ammonium in calcification.
dc.description.sponsorshipDominique Desgré for coral maintenance, Alexander Venn for comments, Christine Ferrier-Pagès for kindly revising the text of the manuscript, and Renaud Grover for fruitful discussions
dc.description.sponsorshipSupported by the Government of the Principality of Monaco and baseline funding from King Abdullah University of Science and Technology (KAUST) to MA. The funding body played no role in the design, collection, analysis, or interpretation of the data; the writing of the manuscript; or the decision to submit the manuscript for publication.
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttps://link.springer.com/10.1007/s00338-022-02256-5
dc.rightsArchived with thanks to Coral Reefs
dc.subjectAmmonium transporters
dc.subjectScleractinian corals
dc.subjectDifferential gene expression
dc.subjectImmunolocalization
dc.subjectCalcification
dc.subjectBiomineralization
dc.titleSpiAMT1d: molecular characterization, localization, and potential role in coral calcification of an ammonium transporter in Stylophora pistillata
dc.typeArticle
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.identifier.journalCoral Reefs
dc.rights.embargodate2023-04-13
dc.eprint.versionPost-print
dc.contributor.institutionMarine Biology Department, Centre Scientifique de Monaco (CSM), 8 Quai Antoine 1er, 98000, Monaco City, Monaco
dc.contributor.institutionSorbonne Université, Collège Doctoral, 75005, Paris, France
kaust.personAranda, Manuel


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