A structural homologue of the plant receptor D14 mediates responses to strigolactones in the fungal phytopathogen Cryphonectria parasitica
de Saint Germain, Alexandre
Le Bris, Philippe
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Environmental Science and Engineering Program
King Abdullah University of Science and Technology Division of Biological and Environmental Science and Engineering Thuwal 23955-6900 Saudi Arabia
Permanent link to this recordhttp://hdl.handle.net/10754/675366
MetadataShow full item record
AbstractStrigolactones (SLs) are plant hormones and important signaling molecules required to promote the arbuscular mycorrhizal (AM) symbiosis. While in plants an α/β-hydrolase, DWARF14 (D14), was shown to act as a receptor that binds and cleaves SLs, the fungal receptor for SLs is unknown. Since AM fungi are currently not genetically tractable, in this study, we used the fungal pathogen Cryphonectria parasitica for which gene deletion protocols exist, as a model, as we have previously shown that it responds to SLs. By means of computational, biochemical and genetic analyses we identified a D14 structural homologue, CpD14. Molecular homology modelling and docking support the prediction that CpD14 interacts with and hydrolyses SLs. The recombinant CpD14 protein shows α/β hydrolytic activity in vitro against the SLs synthetic analogue GR24; its enzymatic activity requires an intact Ser/His/Asp catalytic triad. CpD14 expression in the d14-1 loss-of-function Arabidopsis thaliana line did not rescue the plant mutant phenotype. However, gene inactivation by knock-out homologous recombination reduced fungal sensitivity to SLs. These results indicate that CpD14 is involved in SLs responses in C. parasitica and strengthen the role of SLs as multifunctional molecules acting in plant microbe-interactions.
CitationFiorilli, Forgia, M., de Saint Germain, A., D’Arrigo, G., Cornu, D., Le Bris, P., Al-Babili, S., Cardinale, F., Prandi, C., Spyrakis, F., Boyer, F., Turina, M., & Lanfranco, L. (2022). A structural homologue of the plant receptor D14 mediates responses to strigolactones in the fungal phytopathogen Cryphonectria parasitica. New Phytologist. Portico. https://doi.org/10.1111/nph.18013
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- The computational-based structure of Dwarf14 provides evidence for its role as potential strigolactone receptor in plants.
- Authors: Gaiji N, Cardinale F, Prandi C, Bonfante P, Ranghino G
- Issue date: 2012 Jun 19
- Identification of genes involved in fungal responses to strigolactones using mutants from fungal pathogens.
- Authors: Belmondo S, Marschall R, Tudzynski P, López Ráez JA, Artuso E, Prandi C, Lanfranco L
- Issue date: 2017 May
- Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3.
- Authors: Zhao LH, Zhou XE, Yi W, Wu Z, Liu Y, Kang Y, Hou L, de Waal PW, Li S, Jiang Y, Scaffidi A, Flematti GR, Smith SM, Lam VQ, Griffin PR, Wang Y, Li J, Melcher K, Xu HE
- Issue date: 2015 Nov
- Localized expression of the Dwarf14-like2a gene in rice roots on infection of arbuscular mycorrhizal fungus and hydrolysis of rac-GR24 by the encoded protein.
- Authors: Sisaphaithong T, Yanase M, Mano T, Tanabe S, Minami E, Tanaka A, Hata S, Kobae Y
- Issue date: 2021 Dec 2
- Evolution of strigolactone receptors by gradual neo-functionalization of KAI2 paralogues.
- Authors: Bythell-Douglas R, Rothfels CJ, Stevenson DWD, Graham SW, Wong GK, Nelson DC, Bennett T
- Issue date: 2017 Jun 29