Structure and Functional Capacity of a Benzene-mineralizing, Nitrate-reducing Microbial Community
AuthorsEziuzor, Samuel C.
Corrêa, Felipe Borim
da Rocha, Ulisses Nunes
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/675002
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Abstract<h4>Aims</h4>How benzene is metabolized by microbes under anoxic conditions is not fully understood. Here, we studied the degradation pathways in a benzene-mineralizing, nitrate-reducing enrichment culture.<h4>Methods and results</h4>Benzene mineralization was dependent on the presence of nitrate and correlated to enrichment of a Peptococcaceae phylotype only distantly related to known anaerobic benzene degraders of this family. Its relative abundance decreased after benzene mineralization had terminated, while other abundant taxa - Ignavibacteriaceae, Rhodanobacteraceae and Brocadiaceae - slightly increased. Generally, the microbial community remained diverse despite amendment of benzene as single organic carbon source, suggesting complex trophic interactions between different functional groups. A subunit of the putative anaerobic benzene carboxylase (AbcA) previously detected in Peptococcaceae was identified by metaproteomic analysis suggesting that benzene was activated by carboxylation. Detection of proteins involved in anaerobic ammonium oxidation (anammox) indicates that benzene mineralization was accompanied by anammox, facilitated by nitrite accumulation and the presence of ammonium in the growth medium.<h4>Conclusions</h4>The results suggest that benzene was activated by carboxylation and further assimilated by a novel Peptococcaceae phylotype.<h4>Significance and impact of the study</h4>The results confirm the hypothesis that Peptococcaceae are important anaerobic benzene degraders.
CitationEziuzor, S. C., Corrêa, F. B., Peng, S., Schultz, J., Kleinsteuber, S., da Rocha, U. N., … Vogt, C. (2022). Structure and Functional Capacity of a Benzene-mineralizing, Nitrate-reducing Microbial Community. Journal of Applied Microbiology. doi:10.1111/jam.15443
SponsorsThis work was supported by the German Academic Exchange Service (DAAD) funding SCE, the Helmholtz Association (Germany) through the Young Investigator Group [VH-NG-1248] funding UNR and FBC, and the European regional development funds (EFRE—Europe Funds Saxony) plus the Helmholtz Association. Open access funding enabled and organized by ProjektDEAL.
JournalJournal of Applied Microbiology
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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