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    Quantitative and Multiplex Detection of Extracellular Vesicle-derived microRNA via Rolling Circle Amplification within Encoded Hydrogel Microparticles

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    Name:
    Adv Healthcare Materials - 2022 - Al‐Sulaiman - Quantitative and Multiplex Detection of Extracellular Vesicle‐derived.pdf
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    1.902Mb
    Format:
    PDF
    Description:
    Accepted manuscript
    Embargo End Date:
    2023-01-14
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    Type
    Article
    Authors
    Al-Sulaiman, Dana cc
    Juthani, Nidhi cc
    Doyle, Patrick S. cc
    KAUST Department
    Division of Physical Science and Engineering King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia
    Date
    2022-01-14
    Embargo End Date
    2023-01-14
    Submitted Date
    2021-10-27
    Permanent link to this record
    http://hdl.handle.net/10754/674959
    
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    Abstract
    Extracellular vesicle-derived microRNA (EV-miRNA) represent a promising cancer biomarker for disease diagnosis and monitoring. However, existing techniques to detect EV-miRNA rely on complex, bias-prone strategies and preprocessing steps, making absolute quantification highly challenging. This work demonstrates the development and application of a method for quantitative and multiplex detection of EV-miRNA, via rolling circle amplification within encoded hydrogel particles. By a one-pot extracellular vesicle (EV) lysis and microRNA capture step, we avoid the bias and losses associated with standard RNA extraction techniques. The system offers a large dynamic range (3 orders of magnitude), ease of multiplexing, and a limit of detection down to 2.3 zmol (46 aM), demonstrating its utility in clinical applications based on liquid biopsy tests. Furthermore, orthogonal measurements of EV concentrations coupled with the direct, absolute quantification of miRNA in biological samples results in quantitative measurements of miRNA copy numbers per volume sample, and per extracellular vesicle. This article is protected by copyright. All rights reserved.
    Citation
    Al-Sulaiman, D., Juthani, N., & Doyle, P. S. (2022). Quantitative and Multiplex Detection of Extracellular Vesicle-derived microRNA via Rolling Circle Amplification within Encoded Hydrogel Microparticles. Advanced Healthcare Materials, 2102332. doi:10.1002/adhm.202102332
    Publisher
    Wiley
    Journal
    Advanced Healthcare Materials
    DOI
    10.1002/adhm.202102332
    PubMed ID
    35029040
    Additional Links
    https://onlinelibrary.wiley.com/doi/10.1002/adhm.202102332
    ae974a485f413a2113503eed53cd6c53
    10.1002/adhm.202102332
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