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dc.contributor.authorJesús, León-Ruiz
dc.contributor.authorAnnie, Espinal-Centeno
dc.contributor.authorBlilou, Ikram
dc.contributor.authorBen, Scheres
dc.contributor.authorMario, Arteaga-Vázquez
dc.contributor.authorCruz-Ramirez, Luis Alfredo
dc.date.accessioned2022-01-12T12:10:41Z
dc.date.available2022-01-12T12:10:41Z
dc.date.issued2022-01-07
dc.identifier.citationJesús, L.-R., Annie, E.-C., Ikram, B., Ben, S., Mario, A.-V., & Alfredo, C.-R. (2022). RETINOBLASTOMA RELATED (RBR) interaction with key factors of the RNA-directed DNA methylation (RdDM) pathway. doi:10.1101/2022.01.06.474281
dc.identifier.doi10.1101/2022.01.06.474281
dc.identifier.urihttp://hdl.handle.net/10754/674923
dc.description.abstractSummaryTransposable elements and other repetitive elements are silenced by the RNA-directed DNA methylation pathway (RdDM). In RdDM, POLIV-derived transcripts are converted into double stranded RNA (dsRNA) by the activity of RDR2 and subsequently processed into 24 nucleotide short interfering RNAs (24 -nt siRNAs) by DCL3. 24-nt siRNAs are recruited by AGO4 and serve as guides to direct AGO4 - siRNA complexes to chromatin bound POLV-derived transcripts generated from the template/target DNA. The interaction between POLV, AGO4, DMS3, DRD1, RDM1 and DRM2 promotes DRM2-mediated $\textit{de novo}$ DNA methylation.The Arabidopsis Retinoblastoma protein homolog is a master regulator of cell cycle, stem cell maintenance and development. $\textit{In silico}$ exploration of RBR protein partners revealed that several members of the RdDM pathway contain a motif that confers high affinity binding to RBR, including the largest subunits of POLIV and POLV (NRPD1 and NRPE1), the shared second largest subunit of POLIV and POLV (NRPD/E2), RDR1, RDR2, DCL3, DRM2 and SUVR2. We demonstrate that RBR binds to DRM2, DRD1 and SUVR2. We also report that seedlings from loss -of-function mutants in RdDM and in $\textit{RBR}$ show similar phenotypes in the root apical meristem. Furthermore, we show that RdDM and SUVR2 targets are up-regulated in the $\textit{35S
dc.description.sponsorshipWe wish to thank Vicki Chandler, Steve Jacobsen, Fred Berger and Pauline Jullien for sharing published plant materials. We also thank Dr. Juan Caballero-Pérez for initial advice on bioinformatics. J L-R (CVU 858608) was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) with a PhD Fellowship. A C-R was supported by EMBO-ALTF 1114-2006 and CONACYT 000000000092916 grants. M A-V was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) grants 158550 and A1-S-38383 and Newton Fund of the Royal Society grant NA150181.
dc.publisherCold Spring Harbor Laboratory
dc.relation.urlhttp://biorxiv.org/lookup/doi/10.1101/2022.01.06.474281
dc.relation.urlhttps://www.biorxiv.org/content/biorxiv/early/2022/01/06/2022.01.06.474281.full.pdf
dc.rightsArchived with thanks to Cold Spring Harbor Laboratory
dc.subjectRdDM
dc.subjectde novo DNA methylation
dc.subjectRETINOBLASTOMA
dc.subjectDevelopment
dc.subject42 epigenetics
dc.titleRETINOBLASTOMA RELATED (RBR) interaction with key factors of the RNA-directed DNA methylation (RdDM) pathway
dc.typePreprint
dc.contributor.departmentBiological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955 Saudi Arabia.
dc.eprint.versionPre-print
dc.contributor.institutionLaboratory of Molecular and Developmental Complexity at Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, (CINVESTAV-IPN), 36590 Irapuato, México.
dc.contributor.institutionLaboratory of Molecular Biology, Department of Plant Sciences, Wageningen University, Wageningen, the Netherlands.
dc.contributor.institutionGroup of Epigenetics and Developmental Biology, Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, 91090, Xalapa, México.
kaust.personIkram, Blilou
refterms.dateFOA2022-01-12T12:11:53Z


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