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dc.contributor.authorLi, Mo
dc.contributor.authorRamos Mandujano, Gerardo
dc.date.accessioned2021-12-09T06:05:45Z
dc.date.available2021-12-09T06:05:45Z
dc.date.issued2021-12-08
dc.identifier.citationLi, M., & Ramos-Mandujano, G. (2021). MAVRICS: A Robust and Safe Magnetic Nanoparticle based RNA Extraction Method Compatible with Phenol-chloroform Inactivated Infectious Samples v2. doi:10.17504/protocols.io.b2p8qdrw
dc.identifier.doi10.17504/protocols.io.b2p8qdrw
dc.identifier.urihttp://hdl.handle.net/10754/673960
dc.description.abstractDiagnosis and surveillance of emerging pathogens such as SARS-CoV-2 depend on nucleic acid isolation from clinical and environmental samples. Under normal circumstances, samples would be processed using commercial proprietary reagents in Biosafety 2 (BSL-2) or higher facilities. A pandemic at the scale of COVID-19 has caused a global shortage of proprietary reagents and BSL-2 laboratories to safely perform testing. Therefore, alternative solutions are urgently needed to address these challenges. We developed an open-source method called Magneticnanoparticle-Aided Viral RNA Isolation of Contagious Samples (MAVRICS) that is built upon reagents that are either readily available or can be synthesized in any molecular biology laboratory with basic equipment. Unlike conventional methods, MAVRICS works directly in samples inactivated in acid guanidinium thiocyanate-phenol-chloroform (e.g., TRIzol), thus allowing infectious samples to be handled safely without biocontainment facilities.
dc.publisherZappyLab, Inc.
dc.relation.urlhttps://www.protocols.io/view/mavrics-a-robust-and-safe-magnetic-nanoparticle-ba-b2p8qdrw
dc.rightsThis is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleMAVRICS: A Robust and Safe Magnetic Nanoparticle based RNA Extraction Method Compatible with Phenol-chloroform Inactivated Infectious Samples
dc.typeProtocol
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
kaust.personLi, Mo
kaust.personRamos Mandujano, Gerardo
dc.relation.issupplementtoDOI:10.1002/gch2.202000068
refterms.dateFOA2021-12-09T06:11:01Z
display.relations<b>Is Supplement To:</b><br/> <ul><li><i>[Article]</i> <br/> Ramos-Mandujano, G., Salunke, R., Mfarrej, S., Rachmadi, A. T., Hala, S., Xu, J., … Li, M. (2021). A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples. Global Challenges, 2000068. doi:10.1002/gch2.202000068. DOI: <a href="https://doi.org/10.1002/gch2.202000068" >10.1002/gch2.202000068</a> Handle: <a href="http://hdl.handle.net/10754/663963" >10754/663963</a></a></li></ul>


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This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.