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    Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids

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    Type
    Book Chapter
    Authors
    Mi, Jianing cc
    Moreno, Juan C.
    Alagoz, Yagiz cc
    Liew, Kit Xi
    Balakrishna, Aparna
    Zheng, Xiongjie cc
    Al-Babili, Salim cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    The BioActives Lab
    Plant Science
    Center for Desert Agriculture
    KAUST Grant Number
    CRG4
    CRG5
    Date
    2022-07-21
    Online Publication Date
    2021-11-04
    Print Publication Date
    2022
    Permanent link to this record
    http://hdl.handle.net/10754/673922
    
    Metadata
    Show full item record
    Abstract
    Apocarotenoids (APOs) are a class of carotenoid oxidation products with high structural and functional diversity. Apart from serving as precursors of phytohormones, fungal pheromones and vitamin A, several APOs act as signaling molecules involved in stress response and growth as regulators in plants. To comprehensively profile plant APOs, we established an improved ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical platform. The improved APO analytical platform consists of an optimized sequential APO sample preparation and multiple UHPLC-MS detection methods and was successfully used to identify and quantify multiple subclasses of APOs from tomato fruits. By integrating ultrasound-assisted extraction, solid phase extraction, and chemical derivatization, the improved sequential APOs sample preparation facilitates the simultaneous preparation of different subclasses of APOs from plant materials. In addition, multiple UHPLC-MS detection methods enables high throughput analysis of APOs. Application of this analytical strategy can make important contributions to understanding the function of these compounds and significantly facilitate the elucidation of plant APO metabolism.
    Citation
    Jianing Mi, Juan C. Moreno, Yagiz Alagoz, Kit Xi Liew, Aparna Balakrishna, Xiongjie Zheng, Salim Al-Babili, Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids, Methods in Enzymology, Academic Press, 2021, https://doi.org/10.1016/bs.mie.2021.10.012.
    Sponsors
    This work was supported by baseline funding and a Competitive Research Grant (CRG4 and CRG5) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).
    Publisher
    Academic Press Inc.
    DOI
    10.1016/bs.mie.2021.10.012
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S0076687921004523
    ae974a485f413a2113503eed53cd6c53
    10.1016/bs.mie.2021.10.012
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Book Chapters; Center for Desert Agriculture

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