Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids
Moreno, Juan C.
Liew, Kit Xi
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
The BioActives Lab
Center for Desert Agriculture
Permanent link to this recordhttp://hdl.handle.net/10754/673922
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AbstractApocarotenoids (APOs) are a class of carotenoid oxidation products with high structural and functional diversity. Apart from serving as precursors of phytohormones, fungal pheromones and vitamin A, several APOs act as signaling molecules involved in stress response and growth as regulators in plants. To comprehensively profile plant APOs, we established an improved ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical platform. The improved APO analytical platform consists of an optimized sequential APO sample preparation and multiple UHPLC-MS detection methods and was successfully used to identify and quantify multiple subclasses of APOs from tomato fruits. By integrating ultrasound-assisted extraction, solid phase extraction, and chemical derivatization, the improved sequential APOs sample preparation facilitates the simultaneous preparation of different subclasses of APOs from plant materials. In addition, multiple UHPLC-MS detection methods enables high throughput analysis of APOs. Application of this analytical strategy can make important contributions to understanding the function of these compounds and significantly facilitate the elucidation of plant APO metabolism.
CitationJianing Mi, Juan C. Moreno, Yagiz Alagoz, Kit Xi Liew, Aparna Balakrishna, Xiongjie Zheng, Salim Al-Babili, Ultra-high performance liquid chromatography-mass spectrometry analysis of plant apocarotenoids, Methods in Enzymology, Academic Press, 2021, https://doi.org/10.1016/bs.mie.2021.10.012.
SponsorsThis work was supported by baseline funding and a Competitive Research Grant (CRG4 and CRG5) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).
PublisherAcademic Press Inc.