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    Towards Understanding the Biological Background of Strigolactone Diversity

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    Name:
    PhD Dissertation-Final_Justine Braguy.pdf
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    67.33Mb
    Format:
    PDF
    Description:
    PhD Dissertation
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    Type
    Dissertation
    Authors
    Braguy, Justine cc
    Advisors
    Al-Babili, Salim cc
    Committee members
    Zurbriggen, Matias
    Blilou, Ikram cc
    Bouwmeester, Harro J.
    Lauersen, Kyle J. cc
    Program
    Plant Science
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2021-10
    Embargo End Date
    2022-12-01
    Permanent link to this record
    http://hdl.handle.net/10754/673881
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation will become available to the public after the expiration of the embargo on 2022-12-01.
    Abstract
    Strigolactones (SLs) are a class of plant hormones regulating several aspects of plant growth and development according to nutrient availability, particularly the modulation of root and shoot architectures. Under nutrient deficiency, SLs are abundantly released into the soil to recruit a plant-beneficial partner, arbuscular mycorrhizal fungi (AMF), and establish plant-AMF symbiosis that provides the plant with minerals and water. However, released SLs are also seed germination signals for the root parasitic plants Orobanchacea family and pave their way to the host plants’ roots. “New comers” in the field of plant hormones, their large structural variety intrigues and led to ask why plants produce many different types of SLs. In this work, we generated tools that can help to link the SL structural diversity with their biological function(s). The most common way to evaluate SL activity is based on their ability to be parasitic seeds’ germination stimulants. Despite being the most sensitive assay for SL quantification, parasitic seed-based bioassays are laborious and time-consuming as performed usually manually. Therefore, we developed a detection algorithm, SeedQuant, which identifies and counts germinated and non-germinated seeds 600 times faster than a trained human; thus, reducing the data processing from days down to minutes. To gain quantitative insights in SL perception, depending on the structural diversity, we developed a precise and detailed protocol for the use of a genetically encoded biosensor in Arabidopsis protoplast, StrigoQuant. StrigoQuant takes advantage of the SL-dependent degradation of the repressor protein AtSMXL6 coupled with luciferase reporter proteins. We also tried to adapt this molecular sensor to the rice repressor protein D53, but the use of rice protoplasts made it very challenging. Moreover, to better understand the later steps in SL biosynthesis in vivo, we generated CRISPR/Cas9-based rice mutants and shed light on the biological function of different SLs at the organismal level. MAX1-900 mutants defined the minor role of the canonical SL 4-deoxyorobanchol (4DO) - a major plant SL - in determining rice architecture, while being a crucial contributor to rhizospheric interactions. Finally, we reviewed other strategies to decipher plant signaling pathways in general.
    Citation
    Braguy, J. (2021). Towards Understanding the Biological Background of Strigolactone Diversity. KAUST Research Repository. https://doi.org/10.25781/KAUST-N0998
    DOI
    10.25781/KAUST-N0998
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-N0998
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; PhD Dissertations; Plant Science Program

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