Synthesis and Characterization of ACE2-Based Peptides as Inhibitors & Peptide Epitopes for the Detection of SARS-CoV-2
Sarah Alsawaf - Final Paper
Committee membersHuang, Kuo-Wei
KAUST DepartmentPhysical Science and Engineering (PSE) Division
Embargo End Date2022-12-01
Permanent link to this recordhttp://hdl.handle.net/10754/673877
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Access RestrictionsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis will become available to the public after the expiration of the embargo on 2022-12-01.
AbstractDue to the pandemic, research concerning SARS-CoV-2 became of the utmost importance. In this research, we aimed to find and synthesize a library of peptide epitopes that carry functional properties of the ACE-2 receptor binding to the virus protein for the purpose of creating a therapeutic treatment (i.e. viral inhibition). In order to do this, we used MST to determine binding affinity. After that, we validated the binding properties of our peptide epitopes and applied them as SARSCoV-2 antibody indicators using ELISA. We, then, functionalized gold nanoparticles with the peptide epitopes to assess its utility as a potential SARS-CoV-2 competitive inhibitor. From the set of peptides in the library, P25 showed the most functional properties in both MST and serological ELISA, while P1 successfully conjugated to the gold nanoparticles in different forms (PEG-P1, linker-P1, and mutated P1). Finally, P1 was validated to have antibody binding through sandwich ELISA. In the future, these findings can be applied to inhibit viral activity through drug delivery.
CitationAlsawaf, S. (2021). Synthesis and Characterization of ACE2-Based Peptides as Inhibitors & Peptide Epitopes for the Detection of SARS-CoV-2. KAUST Research Repository. https://doi.org/10.25781/KAUST-51J22