Development, validation, and application of an HPLC-MS/MS method for quantification of oxidized fatty acids in plants
Donizetti de Souza, Gezimar
Sheikh, Arsheed Hussain
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Online Publication Date2021-10-22
Print Publication Date2021-12
Embargo End Date2023-10-22
Permanent link to this recordhttp://hdl.handle.net/10754/673718
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AbstractOxylipins constitute a huge class of compounds produced by oxidation of long-chain unsaturated fatty acids either chemically (by radicals such as reactive oxygen species, ROS) or enzymatically (by lipoxygenases, LOX; cyclooxygenases, COX; or cytochrome P450 pathways). This process generates fatty acids peroxides, which can then be further modified in a broad range to epoxy, hydroxy, keto, ether fatty acids, and also hydrolyzed to generate small aldehydes and alcohols. In general, oxylipins are present in almost all living organisms and have a wide range of signaling, metabolic, physiological, and ecological roles depending on the particular organism and on their structure. In plants, oxylipins have been extensively studied over the past 35 years. However, these studies have focused mainly on the jasmonates and so-called green leaves volatiles. The function of early LOX products (like keto and hydroxy fatty acids) is yet not well understood in plants, where they are mainly analyzed by indirect methods or by GC–MS what requires a laborious sample preparation. Here, we developed and validated a straightforward, precise, accurate, and sensitive method for quantifying oxylipins in plant tissues using HPLC-MS/MS, with a one-step extraction procedure using low amount of plant tissues. We successfully applied this method to quantify the oxylipins in different plant species and Arabidopsis thaliana plants treated with various biotic and abiotic stress conditions.
CitationAlmeida-Trapp, M., Donizetti de Souza, G., Shekhawat, K., Sheikh, A. H., Mithöfer, A., Hirt, H., & Rodrigues-Filho, E. (2021). Development, validation, and application of an HPLC-MS/MS method for quantification of oxidized fatty acids in plants. Journal of Chromatography B, 1186, 123006. doi:10.1016/j.jchromb.2021.123006
SponsorsThe authors thank Prof Dr Sabine Sargo, Dr Andrea Busch and Ms Marek Deckena, for providing the Marchantia samples.
This research has been funded by FAPESP (2010/17925-9) and CAPES-HUMBOLDT (BEX 7611/15-3).
JournalJournal of Chromatography B