Metagenomics-driven rapid diagnosis of an imported fatal case of rare amoebic meningoencephalitis

Guan, Qingtian; Alhuthali, Bushra; Mfarrej, Sara; Halim, Magid Abdel; Almaghrabi, Reem S; Pain, Arnab

Metagenomics-driven rapid diagnosis of an imported fatal case of rare amoebic meningoencephalitis

Files

taab172.pdf (563.95 KB)

License

(https://creativecommons.org/licenses/by-nc/4.0/)

Type

Article

Authors

Guan, Qingtian

Alhuthali, Bushra
Mfarrej, Sara
Halim, Magid Abdel
Almaghrabi, Reem S
Pain, Arnab

KAUST Department

Biological and Environmental Science and Engineering (BESE) Division
Bioscience Program
Pathogen Genomics Laboratory

KAUST Grant Number

BAS/1/1020-01- 01
REI/1/4468-01-01]

Online Publication Date

2021-11-05

Print Publication Date

2022-07-14

Date

2021-11-05

Submitted Date

2021-08-09

Abstract

We identified Naegleria fowleri as the aetiologic agent from cerebrospinal fluid (CSF) of a patient with a travel history to Karachi. We demonstrate the benefit of metagenomics-based rapid diagnosis of primary amoebic meningoencephalitis (PAM) when routine clinical tests fail to provide a definitive diagnosis. We reconstructed the N. fowleri genome.

Citation

Guan, Q., Alhuthali, B., Mfarrej, S., Halim, M. A., Almaghrabi, R. S., & Pain, A. (2021). Metagenomics-driven rapid diagnosis of an imported fatal case of rare amoebic meningoencephalitis. Journal of Travel Medicine. doi:10.1093/jtm/taab172

Acknowledgements

This work was supported by King Abdullah University of Science and Technology Smart Health Initiative fund [REI/1/4468-01-01] to A.P. and R.S.A. and a KAUST faculty baseline fund [BAS/1/1020-01- 01] AP.