The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors
De Bonis, Pasquale
Guerrisi, Maria Giovanna
Farace, Maria Giulia
Ciafre, Silvia Anna
KAUST Grant NumberKUK-I1-012-43
Permanent link to this recordhttp://hdl.handle.net/10754/673084
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AbstractGlioblastoma multiforme (GBM) is the most common and deadliest primary brain tumor, driving patients to death within 15 months after diagnosis (short term survivors, ST), with the exception of a small fraction of patients (long term survivors, LT) surviving longer than 36 months. Here we present deep sequencing data showing that peritumoral (P) areas differ from healthy white matter, but share with their respective frankly tumoral (C) samples, a number of mRNAs and microRNAs representative of extracellular matrix remodeling, TGFß and signaling, of the involvement of cell types different from tumor cells but contributing to tumor growth, such as microglia or reactive astrocytes. Moreover, we provide evidence about RNAs differentially expressed in ST vs LT samples, suggesting the contribution of TGF-β signaling in this distinction too. We also show that the edited form of miR- 376c-3p is reduced in C vs P samples and in ST tumors compared to LT ones. As a whole, our study provides new insights into the still puzzling distinction between ST and LT tumors, and sheds new light onto that "grey" zone represented by the area surrounding the tumor, which we show to be characterized by the expression of several molecules shared with the proper tumor mass.
CitationFazi, B., Felsani, A., Grassi, L., Moles, A., D’Andrea, D., Toschi, N., … Mangiola, A. (2015). The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors. Oncotarget, 6(26), 22526–22552. doi:10.18632/oncotarget.4151
SponsorsThis work was funded by a FIRB grant n.RBAP10KJC5 from the Italian Ministry of University and Education (MIUR) to GM, and by a Fondazione Umberto Veronesi (FUV) grant to SAC. LG and DD have been supported by KAUST Award KUK-I1-012-43.
PublisherIMPACT JOURNALS LLC
PubMed Central IDPMC4673180
CollectionsPublications Acknowledging KAUST Support
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