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    Tracking transience: a method for dynamic monitoring of biological events in Arabidopsis thaliana biosensors

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    Type
    Article
    Authors
    Hines, George
    Modavi, Cyrus
    Jiang, Keni
    Packard, Andrew
    Poolla, Kameshwar
    Feldman, Lewis
    KAUST Grant Number
    93271
    Date
    2015
    Permanent link to this record
    http://hdl.handle.net/10754/673067
    
    Metadata
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    Abstract
    Main conclusion: The activation and level of expression of an endogenous, stress-responsive biosensor (bioreporter) can be visualized in real-time and non-destructively using highly accessible equipment (fluorometer). Biosensor output can be linked to computer-controlled systems to enable feedback-based control of a greenhouse environment. Today’s agriculture requires an ability to precisely and rapidly assess the physiological stress status of plants in order to optimize crop yield. Here we describe the implementation and utility of a detection system based on a simple fluorometer design for real-time, continuous, and non-destructive monitoring of a genetically engineered biosensor plant. We report the responses to heat stress of Arabidopsis thaliana plants expressing a Yellow Fluorescent Protein bioreporter under the control of the DREB2A temperature-sensing promoter. Use of this bioreporter provides the ability to identify transient and steady-state behavior of gene activation in response to stress, and serves as an interface for novel experimental protocols. Models identified through such experiments inform the development of computer-based feedback control systems for the greenhouse environment, based on in situ monitoring of mature plants. More broadly, the work here provides a basis for informing biologists and engineers about the kinetics of bioreporter constructs, and also about ways in which other fluorescent protein constructs could be integrated into automated control systems.
    Citation
    Hines, G., Modavi, C., Jiang, K., Packard, A., Poolla, K., & Feldman, L. (2015). Tracking transience: a method for dynamic monitoring of biological events in Arabidopsis thaliana biosensors. Planta, 242(5), 1251–1261. doi:10.1007/s00425-015-2393-2
    Sponsors
    This work was supported by funding from KAUST (93271) and the National Science Foundation (MCB-313546).
    Publisher
    SPRINGER
    Journal
    PLANTA
    DOI
    10.1007/s00425-015-2393-2
    Additional Links
    http://link.springer.com/10.1007/s00425-015-2393-2
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00425-015-2393-2
    Scopus Count
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    Publications Acknowledging KAUST Support

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