Genomic and metabolic adaptations of biofilms to ecological windows of opportunities in glacier-fed streams
AuthorsBusi, Susheel Bhanu
Kohler, Tyler J
De Staercke, Vincent
de Nies, Laura
Battin, Tom J
KAUST DepartmentRed Sea Research Center (RSRC)
Biological and Environmental Science and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/672824
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AbstractMicroorganisms dominate life in cryospheric ecosystems. In glacier-fed streams (GFSs), ecological windows of opportunities allow complex microbial biofilms to develop and transiently form the basis of the food web, thereby controlling key ecosystem processes. Here, using high-resolution metagenomics, we unravel strategies that allow biofilms to seize this opportunity in an ecosystem otherwise characterized by harsh environmental conditions. We found a diverse microbiome spanning the entire tree of life and including a rich virome. Various and co-existing energy acquisition pathways point to diverse niches and the simultaneous exploitation of available resources, likely fostering the establishment of complex biofilms in GFSs during windows of opportunity. The wide occurrence of rhodopsins across metagenome-assembled genomes (MAGs), besides chlorophyll, highlights the role of solar energy capture in these biofilms. Concomitantly, internal carbon and nutrient cycling between photoautotrophs and heterotrophs may help overcome constraints imposed by the high oligotrophy in GFSs. MAGs also revealed mechanisms potentially protecting bacteria against low temperatures and high UV-radiation. The selective pressure of the GFS environment is further highlighted by the phylogenomic analysis, differentiating the representatives of the genus Polaromonas, an important component of the GFS microbiome, from those found in other ecosystems. Our findings reveal key genomic underpinnings of adaptive traits that contribute to the success of complex biofilms to exploit environmental opportunities in GFSs, now rapidly changing owing to global warming.
CitationBusi, S. B., Bourquin, M., Fodelianakis, S., Michoud, G., Kohler, T. J., Peter, H., … Battin, T. J. (2021). Genomic and metabolic adaptations of biofilms to ecological windows of opportunities in glacier-fed streams. doi:10.1101/2021.10.07.463499
SponsorsWe are thankful for the assistance of Audrey Frachet Bour, Lea Grandmougin, Janine Habier, Laura Lebrun (LCSB) and Emmy Marie Oppliger (EPFL) for laboratory support. We are grateful to Alex Washburne for his feedback on the draft, and we also acknowledge the valuable input from Rashi Halder at the LCSB Sequencing Platform with respect to library preparation. We are equally grateful for the valuable insights into metagenomic processing from Patrick May and Cedric Christian Laczny, and especially Valentina Galata with the python scripts and Snakemake workflows. The computational analyses presented in this paper were carried out using the HPC facilities at the University of Luxembourg (https://hpc.uni.lu)123 562 .
PublisherCold Spring Harbor Laboratory
Except where otherwise noted, this item's license is described as available under a CC-BY 4.0 International license. (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made bioRxiv preprint doi: https://doi.org/10.1101/2021.10.07.463499; this version posted October 7, 2021. T