DNA transposon expansion is associated with genome size increase in mudminnows
Tegner, Jesper N
Lamatsch, Dunja K
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Division of Biological and Environmental Sciences & Engineering, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia
Permanent link to this recordhttp://hdl.handle.net/10754/672064
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AbstractGenome sizes of eukaryotic organisms vary substantially, with whole genome duplications (WGD) and transposable element expansion acting as main drivers for rapid genome size increase. The two North American mudminnows, Umbra limi and U. pygmaea, feature genomes about twice the size of their sister lineage Esocidae (e.g., pikes and pickerels). However, it is unknown whether all Umbra species share this genome expansion and which causal mechanisms drive this expansion. Using flow cytometry, we find that the genome of the European mudminnow is expanded similarly to both North American species, ranging between 4.5-5.4 pg per diploid nucleus. Observed blocks of interstitially located telomeric repeats in Umbra limi suggest frequent Robertsonian rearrangements in its history. Comparative analyses of transcriptome and genome assemblies show that the genome expansion in Umbra is driven by the expansion of DNA transposon and unclassified repeat sequences without WGD. Furthermore, we find a substantial ongoing expansion of repeat sequences in the Alaska blackfish Dallia pectoralis, the closest relative to the family Umbridae, which might mark the beginning of a similar genome expansion. Our study suggests that the genome expansion in mudminnows, driven mainly by transposon expansion, but not WGD, occurred before the separation into the American and European lineage.
CitationLehmann, R., Kovařík, A., Ocalewicz, K., Kirtiklis, L., Zuccolo, A., Tegner, J. N., … Symonová, R. (2021). DNA transposon expansion is associated with genome size increase in mudminnows. Genome Biology and Evolution. doi:10.1093/gbe/evab228
SponsorsThis study was supported by the Tyrolean funds project with contract Nr. UNI-0404/2015 to RS. The authors are also grateful to the`Excelence projekt PřF UHK 2209/2018` and the Czech Science Foundation (19-03442S) for financial support. RS acknowledges the EuroTech Postdoc Programmewhich is co-funded by the European Commission under its framework programme Horizon 2020, Grant Agreement number 754462. We acknowledge Petr Ráb for discussion on genome size in the Umbra genus, Guillaume Côté for fishing Umbra and Esox in Québec, and we also acknowledge Maria Pichler of UIBK for her technical support.
PublisherOxford University Press (OUP)
JournalGenome Biology and Evolution
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