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dc.contributor.authorJia, Kunpeng
dc.contributor.authorMi, Jianing
dc.contributor.authorAli, Shawkat
dc.contributor.authorOhyanagi, Hajime
dc.contributor.authorMoreno, Juan C.
dc.contributor.authorAblazov, Abdugaffor
dc.contributor.authorBalakrishna, Aparna
dc.contributor.authorBerqdar, Lamis
dc.contributor.authorFiore, Alessia
dc.contributor.authorDiretto, Gianfranco
dc.contributor.authorMartínez, Claudio
dc.contributor.authorde Lera, Angel R.
dc.contributor.authorGojobori, Takashi
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2021-09-30T12:37:10Z
dc.date.available2021-09-30T12:37:10Z
dc.date.issued2021-09-20
dc.date.submitted2021-04-29
dc.identifier.citationJia, K.-P., Mi, J., Ali, S., Ohyanagi, H., Moreno, J. C., Ablazov, A., … Al-Babili, S. (2021). An alternative, zeaxanthin epoxidase-independent abscisic acid biosynthetic pathway in plants. Molecular Plant. doi:10.1016/j.molp.2021.09.008
dc.identifier.issn1674-2052
dc.identifier.pmid34547513
dc.identifier.doi10.1016/j.molp.2021.09.008
dc.identifier.urihttp://hdl.handle.net/10754/672051
dc.description.abstractAbscisic acid (ABA) is an important carotenoid-derived phytohormone that plays essential roles in plant response to biotic and abiotic stresses as well as in various physiological and developmental processes. In Arabidopsis, ABA biosynthesis starts with the epoxidation of zeaxanthin by the ABA DEFICIENT 1 (ABA1) enzyme, leading to epoxycarotenoids, e.g., violaxanthin. The oxidative cleavage of 9-cis-epoxycarotenoids, a key regulatory step catalyzed by 9-CIS-EPOXYCAROTENOID DIOXYGENASE, forms xanthoxin that is converted in further reactions mediated by ABA DEFICIENT 2 (ABA2), ABA DEFICIENT 3 (ABA3), and ABSCISIC ALDEHYDE OXIDASE 3 (AAO3) into ABA. By combining genetic and biochemical approaches, we unravel here an ABA1-independent ABA biosynthetic pathway starting upstream of zeaxanthin. We identified the carotenoid cleavage products, i.e., apocarotenoids, β-apo-11-carotenal, 9-cis-β-apo-11-carotenal, 3-OH-β-apo-11-carotenal, and 9-cis-3-OH-β-apo-11-carotenal as intermediates of this ABA1-independent ABA biosynthetic pathway. Using labeled compounds, we showed that β-apo-11-carotenal, 9-cis-β-apo-11-carotenal, and 3-OH-β-apo-11-carotenal are successively converted into 9-cis-3-OH-β-apo-11-carotenal, xanthoxin, and finally into ABA in both Arabidopsis and rice. When applied to Arabidopsis, these β-apo-11-carotenoids exert ABA biological functions, such as maintaining seed dormancy and inducing the expression of ABA-responsive genes. Indeed, the transcriptomic analysis revealed a high overlap of differentially expressed genes regulated by β-apo-11-carotenoids and ABA, but also suggested that these compounds exert ABA-independent regulatory activities. Taken together, our study identifies a biological function for the common plant metabolites β-apo-11-carotenoids, extends our knowledge about ABA biosynthesis and provides new insights into plant apocarotenoid metabolic networks.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S1674205221003683
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Molecular Plant. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular Plant, [, , (2021-09-20)] DOI: 10.1016/j.molp.2021.09.008 . © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCarotenoids
dc.subjectSeed germination
dc.subjectABA
dc.subjectApocarotenoids
dc.subjectArabidopsis Thaliana.
dc.subjectAba Biosynthesis
dc.subjectUltra-high Performance Liquid Chromatography-mass Spectrometry
dc.subjectAba1
dc.titleAn alternative, zeaxanthin epoxidase-independent abscisic acid biosynthetic pathway in plants
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBiological and Environmental Sciences and Engineering Division, Center for Desert Agriculture, The BioActives Lab, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Science and Engineering (CEMSE) Division
dc.contributor.departmentPlant Science
dc.identifier.journalMolecular Plant
dc.rights.embargodate2022-09-20
dc.eprint.versionPost-print
dc.contributor.institutionState Key Laboratory of Cotton Biology, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Jinming Road, Kaifeng, 475004, China.
dc.contributor.institutionItalian National Agency for New Technologies, Energy and Sustainable Economic Development, 00123, Rome, Italy.
dc.contributor.institutionUniversidade de Vigo, Facultade de Química and CINBIO, 36310, Vigo, Spain.
kaust.personJia, Kunpeng
kaust.personMi, Jianing
kaust.personAli, Shawkat
kaust.personOhyanagi, Hajime
kaust.personMoreno, Juan C.
kaust.personAblazov, Abdugaffor
kaust.personBalakrishna, Aparna
kaust.personBerqdar, Lamis
kaust.personGojobori, Takashi
kaust.personAl-Babili, Salim
dc.date.accepted2021-09-12
refterms.dateFOA2021-09-30T12:39:13Z
dc.date.published-online2021-09-20
dc.date.published-print2021-09


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