Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon-contaminated soils in King George Island, Maritime Antarctica
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ArticleAuthors
Jurelevicius, Diogo
Pereira, Raphael da Silva

Mota, Fabio Faria
Cury, Juliano C.
Oliveira, Ivan Cardoso
Rosado, Alexandre S.

Mason, Olivia U.
Jansson, Janet K.
Seldin, Lucy
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionDate
2021-09-20Online Publication Date
2021-09-20Print Publication Date
2022-01Embargo End Date
2022-09-20Permanent link to this record
http://hdl.handle.net/10754/671956
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Soil samples from a transect from low to highly hydrocarbon-contaminated soils were collected around the Brazilian Antarctic Station Comandante Ferraz (EACF), located at King George Island, Antarctica. Quantitative PCR (qPCR) analysis of bacterial 16S rRNA genes, 16S rRNA gene (iTag), and shotgun metagenomic sequencing were used to characterize microbial community structure and the potential for petroleum degradation by indigenous microbes. Hydrocarbon contamination did not affect bacterial abundance in EACF soils (bacterial 16S rRNA gene qPCR). However, analysis of 16S rRNA gene sequences revealed a successive change in the microbial community along the pollution gradient. Microbial richness and diversity decreased with the increase of hydrocarbon concentration in EACF soils. The abundance of Cytophaga, Methyloversatilis, Polaromonas, and Williamsia was positively correlated (p-value = <.05) with the concentration of total petroleum hydrocarbons (TPH) and/or polycyclic aromatic hydrocarbons (PAH). Annotation of metagenomic data revealed that the most abundant hydrocarbon degradation pathway in EACF soils was related to alkyl derivative-PAH degradation (mainly methylnaphthalenes) via the CYP450 enzyme family. The abundance of genes related to nitrogen fixation increased in EACF soils as the concentration of hydrocarbons increased. The results obtained here are valuable for the future of bioremediation of petroleum hydrocarbon-contaminated soils in polar environments.Citation
Jurelevicius, D., Pereira, R. da S., Mota, F. F., Cury, J. C., Oliveira, I. C., Rosado, A. S., … Seldin, L. (2021). Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon-contaminated soils in King George Island, Maritime Antarctica. Geobiology. doi:10.1111/gbi.12472Sponsors
This study was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, financial code 001), and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). The authors gratefully acknowledge the Earth Microbiome Program (EMP) for the sequencing data presented in this study.Publisher
WileyJournal
GeobiologyPubMed ID
34545693Additional Links
https://onlinelibrary.wiley.com/doi/10.1111/gbi.12472ae974a485f413a2113503eed53cd6c53
10.1111/gbi.12472
Scopus Count
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