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dc.contributor.authorFan, Yong
dc.contributor.authorMin, Zheying
dc.contributor.authorAlsolami, Samhan M.
dc.contributor.authorMa, Zhenglai
dc.contributor.authorZhang, E.
dc.contributor.authorChen, Wei
dc.contributor.authorZhong, Ke
dc.contributor.authorPei, Wendi
dc.contributor.authorKang, Xiangjin
dc.contributor.authorZhang, Puyao
dc.contributor.authorWang, Yongliang
dc.contributor.authorZhang, Yingying
dc.contributor.authorZhan, Linfeng
dc.contributor.authorZhu, Haiying
dc.contributor.authorAn, Chenrui
dc.contributor.authorLi, Rong
dc.contributor.authorQiao, Jie
dc.contributor.authorTan, Tao
dc.contributor.authorLi, Mo
dc.contributor.authorYu, Yang
dc.date.accessioned2021-09-08T07:04:50Z
dc.date.available2021-09-08T07:04:50Z
dc.date.issued2021-09-07
dc.identifier.citationFan, Y., Min, Z., Alsolami, S., Ma, Z., Zhang, E., Chen, W., … Yu, Y. (2021). Generation of human blastocyst-like structures from pluripotent stem cells. Cell Discovery, 7(1). doi:10.1038/s41421-021-00316-8
dc.identifier.issn2056-5968
dc.identifier.doi10.1038/s41421-021-00316-8
dc.identifier.urihttp://hdl.handle.net/10754/671117
dc.description.abstractHuman blastocysts are comprised of the first three cell lineages of the embryo: trophectoderm, epiblast and primitive endoderm, all of which are essential for early development and organ formation. However, due to ethical concerns and restricted access to human blastocysts, a comprehensive understanding of early human embryogenesis is still lacking. To bridge this knowledge gap, a reliable model system that recapitulates early stages of human embryogenesis is needed. Here we developed a three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids are similar with human embryos that were cultured for 8 or 10 days in vitro, in terms of embryonic structures, cell lineages and transcriptomic profiles. In conclusion, we developed a scalable system to mimic human blastocyst development, which can potentially facilitate the study of early implantation failure that induced by developmental defects at early stage.
dc.description.sponsorshipThis work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020700), the National Key R&D Program of China (2019YFA0110804, 2017YFA0105001, 2016YFC1000601, 2018YFA0801400, 2018YFC1003203), the National Natural Science Fundation of China (81925013, 81971381, 81771580, 81571400, 82071723, 81871162), Guangdong Basic and Applied Basic Research Foundation (2021B1515020069) and Outstanding Overseas Returnees Fund of the Peking University Third Hospital (BYSYLXHG2019002). Work in the Li laboratory was supported by KAUST Office of Sponsored Research (OSR), under award number BAS/1/1080- 01. We thank the members of the Li lab, Jinna Xu, Baolei Yuan, Chongwei Bi, Xuan Zhou, Gerardo Ramos Mandujano, Khaled Alsayegh, Yingzi Zhang, Yeteng Tian, and KAUST Core Labs for their generous help in this research
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttps://www.nature.com/articles/s41421-021-00316-8
dc.rightsArchived with thanks to Cell Discovery
dc.titleGeneration of human blastocyst-like structures from pluripotent stem cells
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience
dc.contributor.departmentBioscience Program
dc.identifier.journalCell Discovery
dc.eprint.versionPre-print
dc.contributor.institutionDepartment of Gynecology and Obstetrics, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China
dc.contributor.institutionBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China
dc.contributor.institutionYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
dc.contributor.institutionSchool of Mechanics and Civil Engineering, China University of Mining and Technology, Beijing, China.
dc.contributor.institutionClinical Stem Cell Research Center, Peking University Third Hospital, Beijing, China
dc.identifier.volume7
dc.identifier.issue1
kaust.personAlsolami, Samhan
kaust.personLi, Mo
kaust.grant.numberOSR
refterms.dateFOA2021-09-08T07:06:37Z
kaust.acknowledged.supportUnitBAS
kaust.acknowledged.supportUnitCore Labs
kaust.acknowledged.supportUnitKAUST Core Lab
kaust.acknowledged.supportUnitKAUST Office of Sponsored Research (OSR)
dc.date.published-online2021-09-07
dc.date.published-print2021-12


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