Multiple strategies of plant colonization by beneficial endophytic Enterobacter sp. SA187
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
KAUST Grant NumberBAS/1/1062-01-01
Online Publication Date2021-09-15
Print Publication Date2021-10
Embargo End Date2022-09-01
Permanent link to this recordhttp://hdl.handle.net/10754/670960
MetadataShow full item record
AbstractAlthough many endophytic plant growth-promoting rhizobacteria have been identified, relatively little is still known about the mechanisms by which they enter plants and promote plant growth. The beneficial endophyte Enterobacter sp. SA187 was shown to maintain productivity of crops in extreme agricultural conditions. Here we present that roots of its natural host (Indigofera argentea), alfalfa, tomato, wheat, barley and Arabidopsis are all efficiently colonized by SA187. Detailed analysis of the colonization process in Arabidopsis showed that colonization already starts during seed germination, where seed-coat mucilage supports SA187 proliferation. The meristematic zone of growing roots attracts SA187, allowing epiphytic colonization in the elongation zone. Unlike primary roots, lateral roots are significantly less epiphytically colonized by SA187. Root endophytic colonization was found to occur by passive entry of SA187 at lateral-root bases. However, SA187 also actively penetrates the root epidermis by enzymatic disruption of plant cell wall material. In contrast to roots, endophytic colonization of shoots occurs via stomata, whereby SA187 can actively re-open stomata similarly to pathogenic bacteria. In summary, several entry strategies were identified that allow SA187 to establish itself as a beneficial endophyte in several plant species, supporting it's use as a plant growth-promoting bacterium in agriculture systems. This article is protected by copyright. All rights reserved.
CitationSynek, L., Rawat, A., L’Haridon, F., Weisskopf, L., Saad, M. M., & Hirt, H. (2021). Multiple strategies of plant colonization by beneficial endophytic Enterobacter sp. SA187. Environmental Microbiology. doi:10.1111/1462-2920.15747
SponsorsWe thank to I. Blilou (KAUST) for critical reading of the manuscript. This work was supported by the KAUST project No. BAS/1/1062-01-01 to H.H. and by the Swiss National Science Foundation project No. 31003A_179310 to L.W. We thank to developers of FIJI and Inkscape software
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