• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    The phosphoproteome of rice leaves responds to water and nitrogen supply

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Hamzelou, Sara
    Melino, Vanessa J. cc
    Plett, Darren C. cc
    Kamath, Karthik Shantharam
    Nawrocki, Arkadiusz
    Larsen, Martin R.
    Atwell, Brian J.
    Haynes, Paul A. cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2021
    Embargo End Date
    2022-07-16
    Submitted Date
    2021-05-03
    Permanent link to this record
    http://hdl.handle.net/10754/670394
    
    Metadata
    Show full item record
    Abstract
    The scarcity of freshwater is an increasing concern in flood-irrigated rice, whilst excessive use of nitrogen fertilizers is costly and contributes to environmental pollution. To co-ordinate growth adaptation under prolonged exposure to limited water or excess nitrogen supply, plants employ complex systems for signalling and regulation of metabolic processes. There is limited information on the involvement of one of the most important post-translational modifications (PTMs), protein phosphorylation, in plant adaptation to long-term changes in resource supply. Oryza sativa cv. Nipponbare was grown under two regimes of nitrogen from the time of germination to final harvest. Twenty-five days after germination, water was withheld from half the pots in each nitrogen treatment and low water supply continued for an additional 26 days, while the remaining pots were well watered. Leaves from all four groups of plants were harvested after 51 days in order to test whether phosphorylation of leaf proteins responded to prior abiotic stress events. The dominant impact of these resources is exerted in leaves, where PTMs have been predicted to occur. Proteins were extracted and phosphopeptides were analysed by nanoLC-MS/MS analysis, coupled with label-free quantitation. Water and nitrogen regimes triggered extensive changes in phosphorylation of proteins involved in membrane transport, such as the aquaporin OsPIP2-6, a water channel protein. Our study reveals phosphorylation of several peptides belonging to proteins involved in RNA-processing and carbohydrate metabolism, suggesting that phosphorylation events regulate the signalling cascades that are required to optimize plant response to resource supply.
    Citation
    Hamzelou, S., Melino, V. J., Plett, D. C., Kamath, K. S., Nawrocki, A., Larsen, M. R., … Haynes, P. A. (2021). The phosphoproteome of rice leaves responds to water and nitrogen supply. Molecular Omics. doi:10.1039/d1mo00137j
    Sponsors
    SH would like to acknowledge scholarship support from Australian Commonwealth Government – International Research Training Program scholarship (iRTP). This work was supported by Macquarie University, and aspects of this research were conducted at the Australian Proteome Analysis Facility.
    Publisher
    Royal Society of Chemistry (RSC)
    Journal
    MOLECULAR OMICS
    DOI
    10.1039/d1mo00137j
    Additional Links
    http://xlink.rsc.org/?DOI=D1MO00137J
    ae974a485f413a2113503eed53cd6c53
    10.1039/d1mo00137j
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division

    entitlement

     
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.