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dc.contributor.authorAl Alwan, Bader
dc.contributor.authorAbuZineh, Karmen
dc.contributor.authorNozue, Shuho
dc.contributor.authorRakhmatulina, Aigerim
dc.contributor.authorAldehaiman, Mansour M.
dc.contributor.authorAl-Amoodi, Asma S.
dc.contributor.authorSerag, Maged F.
dc.contributor.authorAleisa, Fajr A
dc.contributor.authorMerzaban, Jasmeen
dc.contributor.authorHabuchi, Satoshi
dc.date.accessioned2021-07-15T10:49:07Z
dc.date.available2021-07-15T10:49:07Z
dc.date.issued2021-07-14
dc.date.submitted2020-12-21
dc.identifier.citationAl Alwan, B., AbuZineh, K., Nozue, S., Rakhmatulina, A., Aldehaiman, M., Al-Amoodi, A. S., … Habuchi, S. (2021). Single-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling. Communications Biology, 4(1). doi:10.1038/s42003-021-02398-2
dc.identifier.issn2399-3642
dc.identifier.doi10.1038/s42003-021-02398-2
dc.identifier.urihttp://hdl.handle.net/10754/670237
dc.description.abstractAbstractHematopoietic stem/progenitor cell (HSPC) and leukemic cell homing is an important biological phenomenon that occurs through key interactions between adhesion molecules. Tethering and rolling of the cells on endothelium, the crucial initial step of the adhesion cascade, is mediated by interactions between selectins expressed on endothelium to their ligands expressed on HSPCs/leukemic cells in flow. Although multiple factors that affect the rolling behavior of the cells have been identified, molecular mechanisms that enable the essential slow and stable cell rolling remain elusive. Here, using a microfluidics-based single-molecule live cell fluorescence imaging, we reveal that unique spatiotemporal dynamics of selectin ligands on the membrane tethers and slings, which are distinct from that on the cell body, play an essential role in the rolling of the cell. Our results suggest that the spatial confinement of the selectin ligands to the tethers and slings together with the rapid scanning of a large area by the selectin ligands, increases the efficiency of selectin-ligand interactions during cell rolling, resulting in slow and stable rolling of the cell on the selectins. Our findings provide novel insights and contribute significantly to the molecular-level understanding of the initial and essential step of the homing process.
dc.description.sponsorshipWe thank Ms. Ohoud M. Alharbi for the SEM images of KG1a cells. The research reported in this publication was supported by funding from the King Abdullah University of Science and Technology (KAUST) and the KAUST Office of Sponsored Research under Award No. CRG R2 13 MERZ KAUST 1. We would like to thank Ms. Samar A. Rustum and Ms. Umm Habiba for their support in the management of the lab.
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttp://www.nature.com/articles/s42003-021-02398-2
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleSingle-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling
dc.typeArticle
dc.contributor.departmentBioscience Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.identifier.journalCommunications Biology
dc.eprint.versionPublisher's Version/PDF
dc.identifier.volume4
dc.identifier.issue1
kaust.personAl Alwan, Bader
kaust.personAbu-Zineh, Karmen
kaust.personNozue, Shuho
kaust.personRakhmatulina, Aigerim
kaust.personAldehaiman, Mansour
kaust.personAl-Amoodi, Asma
kaust.personSerag, Maged F.
kaust.personAleisa, Fajr A
kaust.personMerzaban, Jasmeen S.
kaust.personHabuchi, Satoshi
kaust.grant.numberCRG R2 13 MERZ KAUST 1
dc.date.accepted2021-06-23
refterms.dateFOA2021-07-15T10:49:57Z
kaust.acknowledged.supportUnitCRG
kaust.acknowledged.supportUnitKAUST Office of Sponsored Research


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.