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dc.contributor.authorGühl, Kerstin
dc.contributor.authorHolmer, Rens
dc.contributor.authorXiao, Ting Ting
dc.contributor.authorShen, Defeng
dc.contributor.authorWardhani, Titis A.K.
dc.contributor.authorGeurts, René
dc.contributor.authorvan Zeijl, Arjan
dc.contributor.authorKohlen, Wouter
dc.date.accessioned2021-07-15T06:09:32Z
dc.date.available2021-07-15T06:09:32Z
dc.date.issued2021-06-28
dc.date.submitted2021-05-15
dc.identifier.citationGühl, K., Holmer, R., Xiao, T. T., Shen, D., Wardhani, T. A. K., Geurts, R., … Kohlen, W. (2021). The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation in Medicago truncatula. Genes, 12(7), 988. doi:10.3390/genes12070988
dc.identifier.issn2073-4425
dc.identifier.doi10.3390/genes12070988
dc.identifier.urihttp://hdl.handle.net/10754/670231
dc.description.abstractNitrogen fixation by rhizobia is a highly energy-demanding process. Therefore, nodule initiation in legumes is tightly regulated. Environmental nitrate is a potent inhibitor of nodulation. However, the precise mechanism by which this agent (co)regulates the inhibition of nodulation is not fully understood. Here, we demonstrate that in Medicago truncatula the lipo-chitooligosaccharideinduced accumulation of cytokinins is reduced in response to the application of exogenous nitrate. Under permissive nitrate conditions, perception of rhizobia-secreted signalling molecules leads to an increase in the level of four cytokinins (i.e., iP, iPR, tZ, and tZR). However, under high-nitrate conditions, this increase in cytokinins is reduced. The ethylene-insensitive mutant Mtein2/sickle, as well as wild-type plants grown in the presence of the ethylene biosynthesis inhibitor 2-aminoethoxyvinyl glycine (AVG), is resistant to the inhibition of nodulation by nitrate. This demonstrates that ethylene biosynthesis and perception are required to inhibit nodule organogenesis under high-nitrate conditions.
dc.description.sponsorshipThis work was supported by NWO-VICI (865.13.001) to R.G and NWO-VENI (863.15.010) to W.K.
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2073-4425/12/7/988
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe effect of exogenous nitrate on LCO signalling, cytokinin accumulation, and nodule initiation in Medicago truncatula
dc.typeArticle
dc.contributor.departmentCenter for Desert Agriculture, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia
dc.identifier.journalGenes
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionLaboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 6708 PB, Wageningen, The, Netherlands
dc.contributor.institutionBioinformatics Group, Department of Plant Sciences, Wageningen University, 6708 PB, Wageningen, The, Netherlands
dc.contributor.institutionDepartment of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany,.
dc.identifier.volume12
dc.identifier.issue7
dc.identifier.pages988
kaust.personXiao, Ting Ting
dc.date.accepted2021-06-24
dc.identifier.eid2-s2.0-85109292039
refterms.dateFOA2021-07-15T06:10:24Z


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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
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